Anti-Human/Monkey PD-1/CD279 Monoclonal Antibody for FC - Q15116

Core Product Specifications and Parameters

Biological Background: PD-1 Function and Localization

• PD-1 is an inhibitory receptor on antigen-activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Related references: PMID:21276005 PMID:31754127 PMID:32184441
• It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Related references: PMID:21276005 PMID:26602187
• Following T-cell receptor (TCR) engagement, PD-1 associates with the TCR-CD3 complex at the immunological synapse and directly inhibits T-cell activation by recruiting PTPN11/SHP-2, leading to dephosphorylation of key proximal signaling molecules. Related references: PMID:32184441
• The PD-1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. Related references: PMID:28951311
• Blockage of the PD-1 pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. Related references: PMID:22658127 PMID:25034862 PMID:25399552
• PD-1 is a glycoprotein containing an immunoglobulin-like domain, disulfide bonds, and undergoes phosphorylation, ubiquitination, and isopeptide bond formation, consistent with its role as a signaling receptor.
• Subcellular localization is primarily at the cell membrane, where it functions as a transmembrane receptor.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize nonspecific binding.
• Validate PD-1 expression in your sample system, as it is induced upon T-cell activation; resting T cells express low levels.
• The fluorophore is compatible with standard 488 nm lasers; ensure compensation settings are optimized in multicolor panels.
• The immunogen corresponds to the extracellular domain (amino acids 25-168), so the antibody should recognize the native cell surface protein; avoid fixation/permeabilization protocols that may alter the epitope unless validated by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated IVD raw materials, including this anti-PD-1 monoclonal antibody for immune checkpoint studies. As your sourcing bridge, we offer optimized antibody pairs, bulk ancillary reagents, and procurement of rare-target raw materials, streamlining your assay development from concept to clinic. Whether you need ready-to-use conjugates or custom antibody sourcing, CamelBio connects you to a reliable supply of critical reagents for oncology and immunology applications.