Anti-Human/Monkey IgD Monoclonal Antibody () for Flow Cytometry - P01880

Core Product Specifications and Parameters

Biological Background: IgD Function and Localization

• Immunoglobulin heavy constant delta (IgD) is the constant region of delta heavy chains, forming membrane-bound or secreted antibodies upon assembly with light chains.
• Membrane-bound IgD (mIgD) serves as the B-cell receptor (BCR) on peripheral B cells, recognizing antigens and triggering clonal expansion and differentiation upon antigen binding. Related references: PMID:20176268 PMID:22158414
• IgD is co-expressed with IgM on most mature peripheral B cells; both isotypes share identical antigen-binding sites generated by V(D)J recombination and affinity matured by somatic hypermutation. Related references: PMID:17576170 PMID:20176268
• Soluble IgD (sIgD) is present in serum at lower concentrations than IgG, IgA, and IgM but higher than IgE, and is a potent inducer of pro-inflammatory cytokines (TNF, IL1B, IL1RN) and other mediators (IL6, IL10, LIF) from monocytes. Related references: PMID:10702483 PMID:11282392 PMID:8774350
• IgD is found both as a transmembrane receptor on the B-cell surface and as a secreted glycoprotein in serum.
• Post-translational modifications include glycosylation and disulfide bonds, and alternative splicing generates membrane-bound and secreted isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 99–384 of human IgD, encompassing the majority of the extracellular domain; this design may recognize both membrane-bound and soluble IgD.
• This antibody is validated for flow cytometry (FC). Optimize antibody titration and use appropriate isotype controls to ensure specificity.
• For intracellular IgD detection, consider fixation and permeabilization protocols compatible with the fluorophore.
• Cross-reactivity with cynomolgus monkey IgD is reported; validate staining in your target model system with appropriate positive and negative controls.
• Protect the antibody conjugate from light during staining and storage to maintain fluorophore brightness.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier, offering this anti-human/monkey IgD monoclonal antibody to support B-cell immunophenotyping and humoral immunity research. We provide a comprehensive range of validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities also cover rare targets, helping manufacturers streamline development from concept to clinic.