Anti-Human/Monkey CD8a Monoclonal Antibody for Flow Cytometry - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• T-cell surface glycoprotein CD8 alpha chain (alternative name: T-lymphocyte differentiation antigen T8/Leu-2) is a single-pass type I membrane protein encoded by the CD8A gene.
• Functions primarily as a coreceptor for MHC class I molecules, interacting simultaneously with the T-cell receptor (TCR) and MHC class I:peptide complexes on antigen-presenting cells.
• Recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling pathways that lead to lymphokine production, motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs).
• Enables CTLs to recognize and eliminate infected cells and tumor cells; in NK cells, CD8A homodimers promote conjugation and lysis of multiple target cells and support survival and differentiation into memory CD8 T-cells.
• Localizes to the cell membrane and can be secreted; expressed as a disulfide-linked alpha/beta heterodimer on thymus-derived T-cells or as a CD8A homodimer on NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• Contains an immunoglobulin-like domain, is glycosylated, palmitoylated, and forms disulfide bonds; molecular weight varies (21-30 kDa) due to alternative splicing and post-translational modifications.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry; titrate the antibody in your specific cell system to determine the optimal concentration for staining.
• The immunogen corresponds to the extracellular region (amino acids 22-182) of human CD8A, which includes the MHC I-binding immunoglobulin domain; this region is likely surface-exposed on live cells.
• Reported cross-reactivity with human and cynomolgus monkey; test cross-reactivity with other non-human primates or species if needed.
• For multicolor flow panels, consider spectral compensation requirements if using this far-red conjugate; ensure instrument configuration matches the dye.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner offering validated monoclonal antibodies, antibody pairs, and ancillary reagents for diagnostic development from concept to clinic. This anti-CD8a antibody joins our extensive portfolio for immune-oncology and T-cell research, available with bulk ordering and custom sourcing support. Let us help you streamline your assay development with reliable, well-characterized raw materials.