Anti-Human/Monkey CD8a Monoclonal Antibody for FC - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8A is a ~25.7 kDa type I transmembrane glycoprotein, also known as Leu-2 or T8/Leu-2, belonging to the immunoglobulin superfamily. Multiple isoforms exist due to alternative splicing.
• Functions as a co-receptor for the T-cell receptor (TCR) by binding to MHC class I molecules on antigen-presenting cells, critical for thymic T-cell selection and peripheral immune surveillance.
• Upon TCR engagement, the cytoplasmic tail of CD8A associates with the Src-family kinase LCK, initiating signaling cascades that drive cytokine production, cytotoxicity, and T-cell proliferation.
• In cytotoxic T-lymphocytes (CTLs), CD8A-MHC class I interaction enables recognition and elimination of virus-infected cells and tumor cells.
• CD8A can also form homodimers on natural killer (NK) cells, promoting conjugate formation, target cell lysis, and survival signaling.
• Expressed predominantly on the surface of CD8+ T-cells as a heterodimer with CD8B; homodimers are found on subsets of NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• Subcellular location: primarily plasma membrane; also secreted as a soluble form following activation.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and palmitoylation, which influence protein folding and membrane anchoring.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–182) of human CD8A, which is expected to recognize the mature, surface-exposed form of the receptor.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your cell type.
• Include appropriate isotype controls and consider Fc receptor blocking to reduce non-specific binding.
• Validate cross-reactivity with cynomolgus monkey CD8a in your specific biological contexts.
• Note that CD8a homodimers on NK cells may require different staining conditions than heterodimers on T-cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD8a antibody supports immune monitoring and cellular phenotyping in flow cytometry, essential for immuno-oncology and infectious disease research. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.