Anti-Human/Monkey CD69 Monoclonal Antibody for Flow Cytometry - Q07108

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 (Cluster of Differentiation 69), also known as AIM, EA1, MLR-3, CLEC2C, GP32/28, and BL-AC/P26, is a type II transmembrane glycoprotein belonging to the C-type lectin family.
• It functions as an early activation marker rapidly expressed on the surface of platelets, T-lymphocytes, and NK cells upon stimulation, playing a central role in immune cell homeostasis.
• CD69 negatively regulates Th17 cell differentiation by interacting with galectin-1 on immature dendritic cells, thereby modulating adaptive immunity. Related references: PMID:24752896
• It promotes the conversion of naive CD4+ T-cells into regulatory T-cells via the S100A8/S100A9 complex, contributing to immune tolerance. Related references: PMID:26296369
• Acting as an oxidized low-density lipoprotein (oxLDL) receptor in CD4+ T-lymphocytes, CD69 negatively regulates inflammation by inducing PDCD1 expression through NFAT activation. Related references: PMID:35930205
• It participates in adipose tissue-derived mesenchymal stem cell-mediated defense against Pseudomonas aeruginosa infection by promoting ERK1 activation and GM-CSF secretion. Related references: PMID:34841721
• CD69 acts as a S1PR1 agonist, facilitating receptor internalization and degradation to negatively regulate chemotactic responses of effector lymphocytes and dendritic cells to sphingosine 1-phosphate. Related references: PMID:37039481
• The protein is predominantly localized to the cell membrane and is expressed on a variety of activated hematopoietic cells, including T-cells, B-cells, NK cells, neutrophils, eosinophils, and platelets.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , eliminating the need for secondary staining steps and reducing assay complexity in flow cytometry.
• Owing to its specificity for an extracellular epitope (amino acids 78–199), it is suitable for surface staining of live, unfixed cells.
• Consider titrating the antibody to determine the optimal concentration for your specific cell type and activation conditions; use appropriate isotype controls for accurate gating.
• The documented cross-reactivity with cynomolgus monkey (Macaca fascicularis) makes this reagent suitable for non-human primate models in preclinical studies.
• When designing multicolor panels, ensure spectral compatibility with other fluorophores to avoid compensation issues.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers and research institutes with integrated access to high-quality IVD raw materials, from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal antibodies, or bulk ancillary reagents for immune cell profiling, our tailored sourcing solutions support your development of flow cytometry-based assays. Contact us to secure this anti-CD69 antibody and explore our full portfolio of rare-target raw materials.