Anti-Human/Monkey CD64 Monoclonal Antibody for Flow Cytometry - P12314

Core Product Specifications and Parameters

Biological Background: CD64 Function and Localization

• CD64 (FCGR1A) is the high-affinity receptor for the Fc region of immunoglobulin gamma (IgG), playing a pivotal role in linking humoral and cellular immunity.
• It mediates IgG effector functions on monocytes, including antibody-dependent cellular cytotoxicity (ADCC) against virus-infected cells.
• The receptor is expressed as a transmembrane glycoprotein on the cell surface.
• It is specifically expressed on monocytes and macrophages.
• CD64 contains immunoglobulin-like domains and belongs to the immunoglobulin superfamily.
• Post-translational modifications include phosphorylation and glycosylation.
• The protein has a predicted molecular weight of approximately 42.6 kDa but may appear as 38–42 kDa due to glycosylation variants.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 16–288 of human CD64, corresponding to the extracellular domain, which likely yields an antibody recognizing the native receptor.
• This antibody is recommended for flow cytometry (FC). The conjugate enables direct detection without secondary reagents.
• Consider optimizing antibody titration and appropriate isotype controls for your specific cell types (e.g., monocytes/macrophages).
• Cross-reactivity with cynomolgus monkey has been reported; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For immune cell receptor studies such as CD64 characterization, we offer validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and rare target sourcing. Our integrated supply chain supports your workflow from discovery to clinical application.