Anti-Human/Monkey CD3 mAb for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Epsilon Chain Function and Localization

• CD3E (also known as T3E, TCRE, IMD18) encodes the CD3 epsilon chain, a core subunit of the T-cell receptor (TCR)-CD3 complex on T lymphocytes critical for adaptive immunity. Related references: PMID:15294938, PMID:15546002, PMID:2470098

• Upon TCR engagement by antigen-presenting cells, CD3 chains transmit signals via conserved immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains; CD3E ITAMs are phosphorylated by Src family kinases LCK and FYN, creating docking sites for ZAP70. Related references: PMID:2470098, PMID:40592325

• Beyond signal transduction, CD3E regulates T-cell development and participates in TCR-CD3 internalization and cell-surface down-regulation through endocytosis motifs in its cytosolic region. Related references: PMID:10384095, PMID:26507128

• CD3E acts as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 binding that prevents ZAP70 interaction, blocking ERK-NFκB signaling and calcium influx. Related references: PMID:38614099, PMID:12110186

• The protein localizes to the cell membrane as a single-pass type I membrane protein with an extracellular immunoglobulin-like domain, a transmembrane helix, and a short cytoplasmic tail containing ITAMs.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–126) covers the extracellular domain of CD3 epsilon, which may favor recognition of native cell-surface CD3 in flow cytometry.
• For optimal staining, titrate the antibody and include appropriate isotype controls; verify performance using relevant sample systems such as human PBMCs or cynomolgus monkey lymphocytes.
• Consider testing across multiple cell types and activation states to confirm specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies for immune-diagnostics. This anti-CD3 antibody supports T-cell analysis applications; our supply chain covers ancillary reagents and custom antibody development for rare targets. As your one-stop partner, we streamline procurement from discovery to clinical-stage diagnostics.