Anti-Human VCAM-1/CD106 Monoclonal Antibody for Flow Cytometry - P19320

Core Product Specifications and Parameters

Biological Background: VCAM-1/CD106 Function and Localization

• VCAM-1 (CD106) is a cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells, crucial for immune surveillance and inflammation.
  Related references: PMID:31310649

• Acts as a major regulator of leukocyte adhesion to the endothelium by binding various integrins.
  Related references: PMID:10209034

• Upon inflammatory activation, VCAM-1 triggers calcium channels and the small GTPase RAC1 to facilitate leukocyte transendothelial migration.
  Related references: PMID:22970700

• Serves as a bone marrow entry checkpoint by providing 'don't-eat-me' signals within MHC class-I presentation contexts.
  Related references: PMID:35210567

• Expressed on inflamed vascular endothelium and on macrophage-like and dendritic cells in both normal and inflamed tissues.

• Subcellularly localizes to the cell membrane and can be secreted.

• Contains immunoglobulin domains, is glycosylated, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 25-698) of human VCAM-1, encompassing the majority of the protein; this design may aid in detecting both membrane-bound and soluble forms.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type, and include appropriate isotype controls and live/dead staining.
• VCAM-1 expression is upregulated on activated endothelial cells; treating endothelial cultures with pro-inflammatory cytokines (e.g., TNF-α) may enhance detection.
• The conjugate is excited by the 488 nm laser and emits in the green channel; ensure proper compensation when used in multicolor panels.
• Validate antibody performance in relevant human cell lines or primary cells known to express VCAM-1 (e.g., HUVECs, THP-1) before applying to experimental samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to provide a comprehensive range of IVD raw materials, including monoclonal antibodies targeting key immunological markers like VCAM-1/CD106. Our supply capabilities encompass validated antibody pairs, optimized conjugates, and bulk ancillary reagents to support assay development from early research through clinical translation. For specialized needs, our sourcing network can help secure rare-target raw materials, streamlining your path to market.