Anti-Human TREM1 (CD354) Monoclonal Antibody for Flow Cytometry - Q9NP99

Core Product Specifications and Parameters

Biological Background: TREM1 (CD354) Function and Localization

• TREM1 (Triggering receptor expressed on myeloid cells 1, also known as TREM-1, CD354) is a cell surface receptor of the immunoglobulin superfamily that plays pivotal roles in both innate and adaptive immunity by amplifying inflammatory responses. Related references: PMID:10799849 PMID:21393102

• Upon binding ligands such as PGLYRP1, HMGB1, or HSP70, TREM1 multimerizes and associates with the adaptor protein TYROBP/DAP12, triggering downstream signaling. Related references: PMID:17568691 PMID:25595774 PMID:29568119

• The activated TREM1-TYROBP complex initiates a SYK-mediated phosphorylation cascade, activating BTK, MAPK1/3, and PLCG, which collectively promote the release of pro-inflammatory cytokines and chemokines from neutrophils and macrophages. Related references: PMID:14656437 PMID:21659545 PMID:17098818

• TREM1 synergistically amplifies inflammatory signals triggered by Toll-like receptors (TLRs) and NOD-like receptors (NLRs), and is critically involved in the pathophysiology of septic shock and atherosclerosis. Related references: PMID:11323674

• In addition to its pro-inflammatory role, TREM1 can act as a decoy receptor, counterbalancing its own signaling by neutralizing ligands.

• TREM1 is predominantly expressed on the cell membrane of myeloid lineage cells (monocytes, macrophages, neutrophils, dendritic cells), with higher expression in adult liver, lung, and spleen compared to fetal tissues; a soluble isoform is also secreted.

• The protein contains an immunoglobulin-like domain, a transmembrane helix, and disulfide bonds, with a calculated molecular weight of approximately 26.4 kDa; alternative splicing yields isoforms with varying masses.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 21–200), which includes the ligand-binding region, suggesting the antibody likely recognizes the native, membrane-bound receptor.
• For flow cytometry, consider using a standard surface staining protocol on live, unfixed human myeloid cells (e.g., PBMCs, monocyte-derived macrophages) and include appropriate isotype and Fc-blocking controls.
• Given the low to moderate expression levels on resting cells, signal sensitivity may vary; optimization of antibody titration and instrument settings is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop supply chain for IVD raw materials, supporting projects from concept to clinic. This anti-human TREM1 monoclonal antibody is a valuable tool for flow cytometry-based studies of innate immunity and inflammatory diseases. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary reagents, and assisted sourcing for rare targets—ensuring a reliable and comprehensive procurement experience for your development needs.