Anti-Human TNFRSF11A Polyclonal Antibody for WB, ELISA - Q9Y6Q6

Core Product Specifications and Parameters

Biological Background: TNFRSF11A Function and Localization

• Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A), also known as RANK, ODFR, and receptor activator of NF-κB, belongs to the TNF receptor family.
• Receptor for TNFSF11/RANKL (TRANCE/OPGL); essential for RANKL-mediated osteoclastogenesis. Related references: PMID:9878548
• Mediates signaling through interactions with EEIG1, promoting NFATC1 transcription and PLCG2 activation.
• Plays a role in regulating interactions between T-cells and dendritic cells.
• Localized at the cell membrane and membrane rafts.
• Ubiquitously expressed with highest levels in skeletal muscle, thymus, liver, colon, small intestine, and adrenal gland.
• Undergoes alternative splicing; carries disulfide bonds, glycosylation, and phosphorylation; features a signal peptide, transmembrane domain, and extracellular cysteine-rich repeats.
• Associated with diseases including osteopetrosis and deafness.

Experimental Guidance and Technical Tips

• For WB, observe a band around 66 kDa (predicted molecular weight) in human, mouse, and rat samples; validate using appropriate positive control tissues such as thymus or skeletal muscle.
• This polyclonal antibody is suitable for ELISA; consider using the recombinant immunogen (amino acids 30–212) as a positive control to optimize assay conditions.
• Due to post-translational modifications (glycosylation, phosphorylation), the observed molecular weight may differ slightly from the predicted 66 kDa; treat samples with phosphatase or deglycosylating enzymes if necessary.
• For cross-reactive studies in mouse and rat, confirm sequence homology in the immunogen region to ensure expected reactivity; the immunogen aligns with human sequence, but cross-reactivity has been supplier-validated.
• Consider blocking endogenous biotin or peroxidases when using sensitive detection systems in tissues with high metabolic activity.

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CamelBio provides diagnostic manufacturers and research labs with a comprehensive one-stop sourcing platform for IVD raw materials, specializing in bone metabolism and osteoimmunology targets. Explore our catalog for validated anti-TNFRSF11A antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to accelerate your assay development.