Anti-Human TIM-3/HAVCR2 Monoclonal Antibody for FC - Q8TDQ0

Core Product Specifications and Parameters

Biological Background: TIM-3/HAVCR2 Function and Localization

• TIM-3 (HAVCR2, CD366) is a cell surface receptor that generally inhibits innate and adaptive immune responses, and regulates macrophage activation; context-dependent stimulating functions have also been reported.
  Related references:
  PMID:24825777, PMID:11823861

• Inhibits T-helper type 1 (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance.
  Related references:
  PMID:14556005

• In CD8+ T-cells, attenuates TCR-induced signaling by blocking NF-kappaB and NFAT activities, reducing IL-2 secretion.
  Related references:
  PMID:24337741, PMID:26492563

• Expressed on Treg cells and inhibits Th17 cell responses.
  Related references:
  PMID:24838857

• Functions as a receptor for LGALS9 (galectin-9), leading to T-cell suppression and apoptosis; however, this receptor function has been challenged.
  Related references:
  PMID:16286920, PMID:24337741, PMID:23555261

• On natural killer (NK) cells, enhances IFN-gamma production but can also suppress cytotoxicity.
  Related references:
  PMID:22323453, PMID:22383801

• Localizes at the cell membrane and cell junctions; expressed in Th1, Treg, dendritic cells, NK cells, and epithelial tissues, with upregulation in chronic HCV and HIV infections.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–200) of human TIM-3, potentially enabling recognition of the native receptor in flow cytometry.
• This antibody is validated for flow cytometry (FC). For other applications (e.g., Western blot, IHC), consider empirical optimization and validation in the relevant sample system.
• Given TIM-3 expression on multiple immune cell types, include appropriate gating strategies and cell-type markers when analyzing complex cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For studies involving immune checkpoint targets like TIM-3/HAVCR2, we provide validated antibody pairs, bulk ancillary reagents, and customized sourcing of rare-target raw materials. Contact us to streamline your procurement and accelerate your immuno-oncology research and assay development.