Anti-Human SYT12 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IV01

Core Product Specifications and Parameters

Biological Background: SYT12 Function and Localization

• Synaptotagmin-12 (SYT12), also known as Synaptotagmin XII (SytXII), is a member of the synaptotagmin family of synaptic vesicle proteins.
• Unlike many other synaptotagmins, SYT12 does not bind Ca²⁺ or phospholipids, suggesting a distinct regulatory mechanism in neurotransmitter release.
• SYT12 enhances spontaneous neurotransmitter release but does not affect induced neurotransmitter release, indicating a role in basal synaptic activity.
• Essential for mossy-fiber long-term potentiation (LTP) in the hippocampus, a process critical for learning and memory.
• Localized to the synaptic vesicle membrane within cytoplasmic secretory vesicles, consistent with its function in synaptic transmission.
• Identified as a phosphoprotein, potentially subject to activity-dependent regulation.
• Contains transmembrane helices and repeat regions, characteristic of synaptotagmin family topology.
• The gene SYT12 encodes a protein with a calculated molecular mass of 46.5 kDa (UniProt), though observed at 47 kDa under experimental conditions.

Experimental Guidance and Technical Tips

• The immunogen is designed against amino acids 95–200 of human SYT12, an internal region; thus, the antibody is expected to detect full-length SYT12.
• For Western Blot (WB), consider using human or mouse brain tissue lysates as positive controls, given SYT12’s expression in the central nervous system and the observed cross-reactivity with mouse.
• For Immunofluorescence/Immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions to preserve synaptic vesicle membrane epitopes.
• In ELISA applications, the recombinant immunogen or native SYT12 can be used for antibody titration and specificity assessment.
• No cross-reactivity data is available for species other than human and mouse; validate reactivity in target species if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable access to IVD raw materials for neurological disorder and synaptic function research. Our anti-SYT12 polyclonal antibody and other synaptic protein antibodies can be sourced alongside validated antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development. For custom requirements or bulk quantities, our sourcing team offers tailored solutions from concept to clinic.