Anti-Human SIGLEC9 Monoclonal Antibody for Flow Cytometry - Q9Y336

Core Product Specifications and Parameters

Biological Background: SIGLEC9 Function and Localization

• Protein identity and isoforms: Sialic acid-binding Ig-like lectin 9 (Siglec-9), also known as CD329 or protein FOAP-9, is encoded by the SIGLEC9 gene. Alternative splicing produces multiple isoforms [Keywords: Alternative splicing].
• Core function: Putative adhesion molecule that mediates sialic-acid-dependent binding to cells, preferentially binding to α2,3- or α2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
• Gene family: Member of the immunoglobulin superfamily and the siglec family of lectins, characterized by extracellular immunoglobulin-like domains.
• Subcellular localization: Localizes to the plasma membrane as a single-pass type I membrane protein.
• Post-translational modifications: Contains disulfide bonds and is N-glycosylated, features critical for structural stability and ligand interaction [Keywords: Disulfide bond, Glycoprotein].
• Tissue specificity: Expressed by peripheral blood leukocytes, specifically neutrophils and monocytes (not eosinophils). Also detected in liver, fetal liver, bone marrow, placenta, spleen, and at lower levels in numerous other tissues like skeletal muscle, brain, lung, thymus, prostate, mammary gland, adrenal gland, colon, trachea, cerebellum, testis, small intestine, and spinal cord [Tissue specificity].
• Additional keywords: Cell adhesion, immunoglobulin domain, lectin, signal, transmembrane, transmembrane helix. These underline its role in immune cell interactions and signaling.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a bright far-red fluorophore optimized for flow cytometry. Determine the optimal staining concentration by titrating in your specific experimental system.
• SIGLEC9 is expressed on neutrophils and monocytes; use human PBMCs or whole blood samples for validation. Ensure adequate Fc receptor blocking to minimize background.
• Be aware that cis interactions with surface sialic acids on the same cell may mask the epitope. Consider testing staining on sialidase-treated versus untreated cells to assess masking effects.
• Include appropriate isotype controls and viability dyes to gate live cells accurately. For intracellular targets, note that this antibody targets the extracellular domain.
• The product is shipped on ice bags to maintain stability; store at recommended temperature upon receipt.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-human SIGLEC9 monoclonal antibody as part of a comprehensive portfolio for immunology, immuno-oncology, and infectious disease research. As a one-stop IVD raw-material sourcing partner, CamelBio provides diagnostic manufacturers, labs, and research institutes with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support every stage from concept to clinic.