ABflo 488 Anti-Human RANKL/CD254 Monoclonal Antibody for Flow Cytometry (Intracellular) - O14788

Core Product Specifications and Parameters

Biological Background: RANKL/CD254 Function and Localization

• RANKL (TNFSF11, TRANCE, OPGL, ODF, CD254) is a member of the tumor necrosis factor (TNF) ligand superfamily, expressed as both a membrane-bound and soluble cytokine.
• It serves as a key osteoclast differentiation and activation factor by binding to TNFRSF11A/RANK and TNFRSF11B/OPG, leading to NFATC1 nuclear translocation and osteoclast-specific gene induction. Related references: PMID:22664871
• Beyond bone remodeling, RANKL enhances dendritic cell-mediated T-cell proliferation and may regulate T-cell/dendritic cell crosstalk in immune responses.
• Alternative splicing and proteolytic cleavage generate multiple isoforms, with observed protein weights of 27, 30, and 35 kDa reflecting potential glycosylation and processing.
• Subcellular localization includes the cell membrane (type II single-pass membrane protein), cytoplasm, and a secreted form, enabling both juxtacrine and paracrine signaling.
• Tissue expression is highest in peripheral lymph nodes, with lower levels in spleen, leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 136–317), covering the receptor-binding region; this ABflo 488 conjugate is validated for intracellular flow cytometry and may require permeabilization buffers.
• For detecting surface RANKL on live cells, titration and fixation-free protocols should be optimized independently, as the antibody is raised against a recombinant protein and may recognize both membrane-bound and soluble forms.
• Given human specificity, use human-derived samples (e.g., activated T cells, osteoblasts, lymph node cells) and confirm cross-reactivity with relevant controls.
• ABflo 488 exhibits spectral properties similar to Alexa Fluor 488; ensure laser and filter compatibility (excitation ~488 nm, emission ~520 nm) for optimal signal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibodies, including this ABflo 488-conjugated anti-RANKL monoclonal antibody, for bone metabolism and immune-oncology research. Our single-source model covers everything from concept to clinic—delivering optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing support to accelerate assay development and manufacturing scale-up.