Anti-Human Pro-Collagen I alpha (COL1A1) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P - P02452

Core Product Specifications and Parameters

Biological Background: Collagen alpha-1(I) chain Function and Localization

• Collagen alpha-1(I) chain, encoded by COL1A1, is the pro-alpha1(I) chain that assembles with pro-alpha2(I) to form type I procollagen, the major fibrillar collagen of the extracellular matrix.
• Type I collagen is a group I collagen, forming fibrils that provide tensile strength to connective tissues; it is most abundant in tendon, ligaments, and bone.
• The protein is secreted into the extracellular space and undergoes mineralization in bone, binding calcium hydroxyapatite to contribute to bone strength.
• Extensive post-translational modifications, including hydroxylation of proline and lysine residues, glycosylation, and phosphorylation, are essential for triple-helix stability and fibril assembly.
• Mutations in COL1A1 are linked to osteogenesis imperfecta (brittle bone disease), Ehlers-Danlos syndrome, osteoporosis, and dwarfism, reflecting its critical structural role.
• The mature protein contains characteristic Gly-X-Y repeats and disulfide-bonded C-terminal propeptide, with a signal peptide directing secretion.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 26-161) of human COL1A1, targeting the propeptide domain; consider that this region may be cleaved in mature collagen fibrils, potentially affecting detection of fully processed forms.
• For western blot (WB), the predicted molecular weight is ~138 kDa; due to extensive cross-linking and post-translational modifications, the observed molecular weight may vary; validate in your sample system using appropriate positive controls (e.g., human fibroblast lysates or bone extracts).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimize antigen retrieval and permeabilization protocols to expose epitopes within the dense extracellular matrix; consider enzymatic digestion to improve antibody penetration.
• This antibody is validated for human reactivity; cross-reactivity with other species is not characterized; if using non-human samples, perform a pilot experiment to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your diagnostic development with a comprehensive portfolio of IVD raw materials, including optimized polyclonal antibodies like this collagen I antibody for connective tissue research. From validated antibody pairs and bulk ancillary reagents to rare target sourcing, we provide one-stop access to critical tools for extracellular matrix and bone biology applications. Partner with CamelBio to streamline your assay development from concept to clinic.