Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit Polyclonal Antibody for WB and ELISA - Q13093

Core Product Specifications and Parameters

Biological Background: Platelet-activating factor acetylhydrolase Function and Localization

• Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress responses. Related references: PMID:10066756, PMID:16371369, PMID:17090529

• Hydrolyzes the ester bond of fatty acyl groups at the sn-2 position of phospholipids, with a preference for short-chain or oxidized long-chain fatty acyl groups. Related references: PMID:10504265, PMID:2040620, PMID:8624782

• Inactivates platelet-activating factor (PAF) to downregulate pro-inflammatory signaling in innate immune cells. Related references: PMID:10066756, PMID:10504265, PMID:11590221

• Prevents accumulation of oxidatively truncated phospholipids in low-density lipoprotein (LDL) particles. Related references: PMID:2040620, PMID:7700381

• Within high-density lipoprotein (HDL) particles, hydrolyzes phospholipid hydroperoxides, contributing to vascular protection. Related references: PMID:17090529

• Catalyzes the release of F2-isoprostanes from membrane phospholipids, serving as biomarkers of cellular oxidative damage. Related references: PMID:16371369

• Secreted into the extracellular space; found in plasma and produced by macrophages. Related references: PMID:11590221, PMID:12821559

• Glycoprotein associated with HDL and LDL; belongs to the phospholipase A2 family, playing a key role in lipid metabolism and inflammatory disease.

Experimental Guidance and Technical Tips

• The immunogen encompasses the mature protein (aa 22–441), covering the catalytic domain; the resulting polyclonal antibody may detect both native and denatured PLA2G7.
• For Western blot, a band around 50 kDa is expected; validate detection in relevant human or mouse sample lysates.
• In ELISA, this antibody may be suitable as a capture or detection reagent; optimization of coating and dilution conditions is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for quantitative assays.
• Cross-reactivity with mouse enables translational studies, but species-specific performance should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials for cardiovascular and inflammation research, supporting your workflow from discovery to validation. In addition to quality-assured anti-PLA2G7 antibodies, our platform offers validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets. Partner with CamelBio to streamline your diagnostic development and research supply needs.