Anti-Human PD-L1/CD274 Monoclonal Antibody for Flow Cytometry - Q9NZQ7

Core Product Specifications and Parameters

Biological Background: PD-L1/CD274 Function and Localization

• PD-L1 (CD274, B7-H1) is a type I transmembrane protein belonging to the immunoglobulin superfamily, sharing structural homology with B7 co‑stimulatory molecules.
• Acts as a ligand for the inhibitory receptor PD‑1 (PDCD1), delivering signals that modulate the activation threshold of T‑cells and limit effector responses. Related references: PMID:11015443 PMID:28813410 PMID:28813417
• Through a yet unidentified activating receptor, PD‑L1 may co‑stimulate T‑cell subsets that predominantly produce interleukin‑10 (IL‑10). Related references: PMID:10581077
• Under hypoxic conditions, PD‑L1 translocates to the nucleus and acts as a transcription coactivator for GSDMC, promoting pyroptosis. Related references: PMID:32929201
• The PD‑L1/PD‑1 pathway is frequently exploited by tumors to attenuate anti‑tumor immunity, and its blockade can reverse T‑cell exhaustion, providing the basis for cancer immunotherapy. Related references: PMID:28813410 PMID:28813417
• Subcellular localization includes the cell membrane, early endosome membrane, recycling endosome membrane, and nucleus; a secreted form also exists.
• Widely expressed in tissues such as heart, skeletal muscle, placenta, and lung; also found on activated immune cells (T‑cells, B‑cells, dendritic cells, monocytes) and various peripheral blood cells.
• The protein undergoes N‑linked glycosylation, contains disulfide bonds, and is subject to ubiquitin‑like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 1‑290) of human PD‑L1, making the antibody suitable for detecting surface‑expressed PD‑L1 in living cells by flow cytometry.
• Titrate the antibody in a concentration range (e.g., 0.1–5 µg/mL) to determine the optimal staining index for your specific cell type and experimental setup.
• Validate antibody specificity using appropriate controls such as PD‑L1‑knockout cells, blocking peptides, or isotype controls to confirm specific binding.
• For applications beyond flow cytometry, the antibody may be tested in other assays (e.g., immunofluorescence) after careful validation in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for IVD raw materials, including this anti‑PD‑L1 monoclonal antibody for immuno‑oncology applications. We supply validated antibody pairs, bulk ancillary reagents, and facilitate the procurement of rare‑target raw materials, supporting manufacturers and laboratories from concept to clinic. Contact us to integrate high‑quality immunoreagents into your diagnostic or research workflows.