Anti-Human PD-L1/CD274 Monoclonal Antibody for Flow Cytometry - Q9NZQ7

Core Product Specifications and Parameters

Biological Background: PD-L1/CD274 Function and Localization

• PD-L1 (CD274, B7-H1) is a type I transmembrane protein of the immunoglobulin superfamily that serves as the ligand for the inhibitory immune checkpoint receptor PD-1.
• It plays a critical role in maintaining immune tolerance by modulating T‑cell activation thresholds and limiting effector responses upon PD‑1 engagement. Related references: PMID:11015443 PMID:28813410 PMID:28813417
• Tumor cells often exploit the PD-1/PD-L1 pathway to suppress anti‑tumor immunity; blocking this interaction can reverse T‑cell exhaustion and restore cytotoxic activity. Related references: PMID:28813410 PMID:28813417
• Through an unknown activating receptor, PD-L1 may also costimulate subsets of T cells that predominantly produce interleukin‑10 (IL‑10). Related references: PMID:10581077
• Under hypoxic conditions, PD-L1 translocates to the nucleus, interacts with phosphorylated STAT3, and acts as a transcription coactivator to promote expression of GSDMC, leading to pyroptosis. Related references: PMID:32929201
• It is localized primarily at the cell membrane and in early/recycling endosome membranes; a secreted form also exists, and nuclear translocation occurs in specific contexts.
• PD‑L1 is highly expressed in heart, skeletal muscle, placenta, and lung, with lower levels in thymus, spleen, kidney, and liver. It is also expressed on activated T‑ and B‑cells, dendritic cells, keratinocytes, and monocytes.
• Structural features include immunoglobulin-like domains, a disulfide bond, N‑glycosylation, and ubiquitination sites.

Experimental Guidance and Technical Tips

• The immunogen encompasses the entire extracellular and transmembrane domains (amino acids 1‑290), suggesting that the antibody likely recognizes the native cell‑surface conformation, supporting its use in flow cytometry.
• A standard titration experiment on PD‑L1‑positive cells is recommended to determine the optimal antibody concentration for your specific experimental system.
• For reliable flow cytometry results, include appropriate isotype controls and live/dead discrimination gating.
• Cross‑reactivity has been confirmed for human samples; performance in other species is not guaranteed and should be validated by the end‑user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high‑quality IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For those working on the PD‑1/PD‑L1 immune checkpoint, we supply monoclonal antibodies like this anti‑PD‑L1 clone, complemented by a broad portfolio for immuno‑oncology assay development. Partner with CamelBio to efficiently source even rare‑target raw materials and advance your project from concept to clinic.