Anti-Human Neurogranin Rabbit Polyclonal Antibody for WB, ELISA - Q92686

Core Product Specifications and Parameters

Biological Background: Neurogranin Function and Localization

• Neurogranin (NRGN), also known as RC3, is a postsynaptic protein that acts as a "third messenger" substrate of protein kinase C (PKC)-mediated molecular cascades during synaptic development and remodeling.
• It binds calmodulin in the absence of calcium, thereby regulating calmodulin availability and downstream calcium/calmodulin-dependent signaling.
• In the human brain, neurogranin is predominantly expressed in the cerebral cortex, specifically in the cell bodies of neurons in layers II-VI and in apical and basal dendrites of pyramidal neurons.
• Notably, neurogranin is absent from dendrites in patients with Alzheimer disease, linking it to neurodegenerative pathology.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, citrullination, and methylation, which may modulate its function and calmodulin-binding affinity.
• As a small calmodulin-binding protein (approx. 8 kDa), neurogranin is a key regulatory hub in synaptic signaling and is implicated in learning and memory processes.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 2-78 of human neurogranin, NP_001119653.1) covers nearly the entire mature protein; the antibody is predicted to recognize full-length neurogranin.
• For WB, neurogranin has a predicted molecular weight of approximately 8 kDa; consider using a high-percentage gel and optimized transfer conditions for low-molecular-weight proteins.
• The antibody cross-reacts with mouse and rat, suggesting conserved epitopes; validate in the relevant species and tissue context.
• As a phosphoprotein, dephosphorylation may affect antibody binding; the use of phosphatase inhibitors during sample preparation could be considered.
• ELISA applications may require optimization of coating antigen and detection protocols to achieve optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For neurogranin and neuroscience-related targets, we offer validated polyclonal antibodies like this anti-human neurogranin antibody, alongside optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.