Anti-Human Neurogranin (NRGN) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q92686

Core Product Specifications and Parameters

Biological Background: Neurogranin Function and Localization

• Neurogranin (NRGN), also known as RC3, is a calmodulin-binding protein predominantly expressed in the brain, particularly in the cerebral cortex and hippocampus.
• It functions as a postsynaptic 'third messenger' substrate of protein kinase C (PKC), playing a key role in synaptic development, plasticity, and remodeling.
• Neurogranin binds calmodulin in the absence of calcium, regulating calcium/calmodulin-dependent signaling pathways and influencing synaptic strength.
• The protein undergoes multiple post-translational modifications, including acetylation, citrullination, methylation, and phosphorylation, which may modulate its activity and localization.
• In cortical tissue, Neurogranin is localized to cell bodies of neurons in layers II–VI and to apical and basal dendrites of pyramidal neurons.
• Notably, Neurogranin is absent from dendrites in patients with Alzheimer disease, suggesting a pathological link to synaptic dysfunction.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 2–78 of human Neurogranin, covering the entire protein. Consider that this region contains phosphorylation, acetylation, and other modification sites that could influence antibody binding under certain experimental conditions.
• For Western blot (WB), Neurogranin has a predicted molecular weight of ~8 kDa; ensure appropriate gel percentage and transfer conditions to retain low-molecular-weight proteins.
• In immunohistochemistry (IHC-P), validation in brain tissue sections is recommended, focusing on cortical layers where expression is highest.
• This antibody cross-reacts with mouse and rat Neurogranin; always validate in the relevant sample system for your specific experiment.
• For ELISA, consider coating with recombinant Neurogranin protein or the immunogen peptide to establish a robust assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a reliable sourcing partner, we supply high-quality antibodies like this anti-Neurogranin monoclonal antibody, along with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Whether you are developing neuroscience diagnostics or studying synaptic pathology, CamelBio streamlines your supply chain with a helpful B2B approach, ensuring you can move from discovery to clinical application efficiently.