Anti-Human Nectin-4 Monoclonal Antibody () for Flow Cytometry - Q96NY8

Core Product Specifications and Parameters

Biological Background: Nectin-4 Function and Localization

• Nectin-4, also known as PVRL4, LNIR, and PRR4, is a cell adhesion molecule of the nectin family that contains immunoglobulin-like domains, a transmembrane helix, and a signal peptide.
• It mediates trans-homophilic and -heterophilic cell–cell adhesion, specifically interacting with nectin-1; it does not serve as a receptor for alpha-herpesvirus entry.
• In the context of microbial infection, Nectin-4 acts as a host cell receptor for measles virus, facilitating viral entry.
• The protein localizes primarily to the cell membrane and adherens junctions, but a secreted isoform also exists, consistent with alternative splicing.
• Nectin-4 is predominantly expressed in placenta; it is absent in normal breast epithelium but is highly expressed in breast carcinoma, suggesting a role in tumor progression.
• Mutations in the NECTIN4 gene are associated with ectodermal dysplasia, a developmental disorder affecting skin, hair, nails, and teeth.
• The canonical full-length protein has a calculated molecular weight of approximately 55 kDa; additional isoforms or cleavage products may yield bands around 24 kDa.

Experimental Guidance and Technical Tips

• The conjugate is suitable for direct flow cytometry on live cells; consider validating performance on known human Nectin-4-expressing lines such as placental cells or breast carcinoma lines.
• Because the immunogen spans the extracellular domain (aa 32–146), the antibody likely recognizes surface-exposed epitopes; be aware that secreted or shed Nectin-4 may also be detected in supernatant or permeabilized samples.
• For flow cytometry, titrate the antibody to determine optimal staining concentration and include an appropriate isotype control.
• Given alternative splicing, confirm which isoform(s) are relevant in your experimental system; the antibody may detect multiple forms.
• This clone cannot be used to block alpha-herpesvirus entry, but it may be employed in measles virus infection studies to monitor receptor expression or viral attachment.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated B2B partner for the IVD industry, CamelBio streamlines raw-material procurement with a focus on Nectin-4 and related targets in cell adhesion, viral entry, and oncology. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare-target raw materials—empowering diagnostic manufacturers to advance from concept to clinic with confidence.