Anti-Human MMP9 Monoclonal Antibody for Intracellular Flow Cytometry - P14780

Core Product Specifications and Parameters

Biological Background: MMP9 Function and Localization

• MMP9, also known as gelatinase B or 92 kDa type IV collagenase, is a zinc-dependent endopeptidase belonging to the matrix metalloproteinase (MMP) family.
• It plays an essential role in the local proteolysis of extracellular matrix components and in leukocyte migration. Related references: PMID:12879005, PMID:1480034, PMID:2551898
• MMP9 specifically cleaves KiSS1 at a Gly-|-Leu bond. Related references: PMID:12879005
• Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Related references: PMID:32883094
• Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Related references: PMID:1480034
• Degrades fibronectin but not laminin or Pz-peptide.
• May play a role in bone osteoclastic resorption (By similarity).
• Detected in neutrophils and produced by normal alveolar macrophages and granulocytes; localized to the extracellular space and matrix.
• Contains calcium and zinc binding sites, disulfide bonds, and undergoes proteolytic activation from the zymogen form.

Experimental Guidance and Technical Tips

• The immunogen spans the majority of the mature MMP9 sequence (amino acids 20–707), suggesting the antibody may recognize both pro- and active forms of the enzyme. For intracellular flow cytometry, use appropriate permeabilization buffers to ensure antibody access to the intracellular compartment.
• Since cross-reactivity is listed only for human MMP9, validate specificity in your sample system of interest, especially when working with closely related MMP family members.
• For optimal results, titrate the antibody concentration and include appropriate isotype controls in your flow cytometry experiments.
• This product is recommended for research use and its performance in diagnostic applications should be independently validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and laboratories from concept to clinic. This anti-MMP9 monoclonal antibody is well-suited for research in matrix biology, inflammation, and cancer, and we can assist with bulk orders, validated antibody pairs, and ancillary reagents tailored to your workflow. Our team also specializes in sourcing rare-target raw materials and providing technical consulting to accelerate your assay development.