Anti-Human MICA/MICB Monoclonal Antibody for Flow Cytometry - Q29983 / Q29980

Core Product Specifications and Parameters

Biological Background: MICA/MICB Function and Localization

• MICA (MHC class I polypeptide-related sequence A) is a stress-induced ligand for the activating receptor NKG2D (KLRK1) expressed on NK cells, γδ T cells, and CD8+ αβ T cells. Related references: PMID:11491531, PMID:11777960
• Upon cellular stress such as viral/bacterial infection or DNA damage, MICA is upregulated and serves as a danger signal, triggering NK cell-mediated cytotoxicity and cytokine production. Related references: PMID:10426993
• MICA is widely expressed except in the central nervous system, with high levels in gastric epithelium, monocytes, keratinocytes, endothelial cells, and fibroblasts; it is also overexpressed in many epithelial tumors.
• Subcellularly, MICA localizes to the cell membrane and cytoplasm.
• The protein is glycosylated, palmitoylated, and can undergo alternative splicing; it contains an immunoglobulin-like domain and a transmembrane region.
• MICB is a closely related family member with similar function and expression as a ligand for NKG2D; this antibody recognizes both MICA and MICB.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , enabling one-step flow cytometry staining without secondary detection reagents.
• Titrate the antibody to determine the optimal dilution for your specific cell type and experimental conditions.
• Because MICA/MICB expression is stress-inducible, consider using positive stimuli (e.g., heat shock, DNA‑damaging agents) to enhance detection in cultured cells.
• Include appropriate isotype controls and use freshly prepared cells to minimize non‑specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-MICA/MICB conjugate is part of our immune-oncology and cellular stress monitoring range, and our team can assist with sourcing rare targets or scaling up your raw-material needs to help bridge concept-to-clinic development.