Anti-Human LIFR/CD118 Monoclonal Antibody for Flow Cytometry - P42702

Core Product Specifications and Parameters

Biological Background: LIFR Function and Localization

• LIFR encodes the leukemia inhibitory factor receptor (CD118), a signal-transducing subunit that forms complexes with IL6ST/gp130 or CNTFR. It mediates signaling for cytokines including LIF, OSM, CNTF, CTF1, CLCF1, and the CRLF1-CLCF1 heterodimer. Related references: PMID:11285233, PMID:11294841, PMID:1542794
• Ligand binding induces heterodimerization of LIFR with gp130, activating associated JAK tyrosine kinases. This leads to phosphorylation of gp130 and subsequent recruitment and activation of STAT transcription factors. Related references: PMID:9030543
• The receptor can also inhibit LIF bioactivity through competitive binding, blocking the interaction of LIF with its signaling receptors.
• LIFR engages site 3 of cytokine ligands, as shown in structural studies. Related references: PMID:36930708
• Subcellular localization includes the cell membrane and secreted forms; the protein is post-translationally modified by glycosylation, phosphorylation, and disulfide bond formation.
• Alternative splicing generates multiple isoforms, and mutations in LIFR are associated with Stüve-Wiedemann syndrome (SWS) and other skeletal dysplasias.
• The full-length receptor has a molecular weight of approximately 124 kDa and contains an extracellular domain, a single transmembrane helix, and a cytoplasmic tail.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the LIFR extracellular domain (aa 45–833), encompassing the cytokine-binding region. This design may enable recognition of both membrane-bound and soluble receptor forms.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions. Use appropriate isotype controls and Fc blocking reagents to minimize background.
• Given the confirmed human cross-reactivity, validate the antibody in the relevant human sample system. Performance in other species has not been assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. This anti-LIFR/CD118 product serves research into cytokine signaling and receptor biology. Our supply chain capabilities enable reliable access to rare-target reagents and customized sourcing solutions to accelerate your development from concept to clinic.