Anti-Human LAMP2 Monoclonal Antibody () for Flow Cytometry - P13473

Core Product Specifications and Parameters

Biological Background: LAMP2 Function and Localization

• LAMP2 is a heavily glycosylated lysosomal membrane protein belonging to the LAMP family, characterized by a large luminal domain, a single transmembrane helix, and a short cytoplasmic tail.
• Functions as a critical regulator of lysosomal pH by directly inhibiting the proton channel TMEM175, thereby facilitating lysosomal acidification for optimal hydrolase activity. Related references: PMID:37390818
• Plays an essential role in chaperone-mediated autophagy (CMA), binding cytosolic proteins such as GAPDH, GPX4, NLRP3, and MLLT11, and targeting them for lysosomal degradation downstream of HSC70 recognition. Related references: PMID:11082038 PMID:18644871 PMID:24880125 PMID:36586411
• Required for macroautophagy by mediating the fusion of autophagosomes with lysosomes, likely through the recruitment of STX17 to autophagosomes. Related references: PMID:27628032
• Contributes to adaptive immunity by enabling efficient MHC class II-mediated presentation of exogenous antigens via its role in lysosomal protein degradation. Related references: PMID:15894275 PMID:20518820
• Alternative splicing produces three main isoforms (LAMP-2A, LAMP-2B, and LAMP-2C) with distinct tissue distribution patterns; LAMP-2A is highly expressed in placenta, lung, and liver, while LAMP-2B and LAMP-2C are more broadly expressed across tissues. Related references: PMID:26856698 PMID:7488019
• Localizes predominantly to the lysosome membrane, and is also found on endosome, cell, and autophagosome membranes.
• Associated with keywords such as alternative splicing, autophagy, glycogen storage disease, glycoprotein, disulfide bond, and transmembrane helix, reflecting its structural and functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29–375 of human LAMP2, encompassing the entire luminal domain; thus, the antibody likely recognizes a conserved epitope present across all splice isoforms.
• Designed for intracellular flow cytometry; ensure proper cell permeabilization to allow access to the lysosomal membrane protein.
• For surface staining, note that LAMP2 can shuttle to the plasma membrane, but permeabilization will detect total LAMP2. Validate in your cell system to distinguish surface versus intracellular populations.
• As with all flow cytometry applications, titrate the antibody to determine the optimal concentration for your specific sample type and staining conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as your comprehensive sourcing partner for IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-LAMP2 antibody supports research into lysosomal biology, autophagy, and immune antigen presentation, areas critical for diagnostic and therapeutic development. Leverage our supply chain expertise to secure even rare target raw materials, helping you accelerate every stage from concept to clinic.