Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P0CG04

Core Product Specifications and Parameters

Biological Background: Human Lambda Light Chain Function and Localization

• The immunoglobulin lambda light chain is a critical component of antibodies, pairing with heavy chains to form the antigen‑binding site; the constant region is encoded by the IGLC1 gene and is subject to V‑(D)‑J rearrangement and somatic hypermutation for affinity maturation. Related references: PMID:17576170 PMID:20176268
• Membrane‑bound immunoglobulins serve as B‑cell receptors that, upon antigen binding, trigger clonal expansion and differentiation into plasma cells; secreted forms mediate humoral effector functions, including pathogen neutralization and clearance. Related references: PMID:20176268 PMID:22158414
• Alternative names include Ig lambda chain C region MGC and Ig lambda‑1 chain C region; free light chains, known as Bence‑Jones proteins, can be detected in urine under pathological conditions.
• Subcellular localization is both secreted and anchored to the cell membrane of B lymphocytes, allowing participation in both recognition and effector stages of adaptive immunity.
• The protein contains a conserved immunoglobulin domain stabilized by an intramolecular disulfide bond and has a calculated molecular weight of approximately 11.3 kDa (UniProt: P0CG04).
• As part of the humoral immune system, lambda light chain expression is a hallmark of B‑cell lineage commitment and is often used as a marker in immunophenotyping and clonality assessment.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–106, encompassing the entire constant domain; this design provides strong reactivity with native human lambda light chain in both free and heavy‑chain‑associated forms.
• For WB under reducing conditions, the full‑length light chain (when associated with heavy chain) may appear at ~25 kDa; a band at ~11 kDa can be observed when detecting the recombinant constant region or free light chain. Validate the banding pattern in your sample system.
• In IF‑P and IHC‑P, expect membrane and/or cytoplasmic staining in mature B cells and plasma cells; appropriate permeabilization may reveal intracellular staining in antibody‑secreting cells.
• For ELISA, the antibody can be used as a capture or detection reagent for lambda light chain quantification in serum, cell culture supernatant, or urine. Consider pairing with an anti‑kappa antibody for light chain ratio analysis.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials. This anti‑human lambda light chain monoclonal antibody exemplifies our commitment to providing validated antibody clones, optimized for applications from basic immunoglobulin research to clinical immunoassay development. Beyond individual antibodies, our one‑stop sourcing includes matched antibody pairs, bulk ancillary reagents, and rare‑target procurement, all supported by technical consultation to accelerate your project from concept to clinic.