Anti-Human Lambda Light Chain Monoclonal Antibody () for Flow Cytometry - P0CG04

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Lambda Constant 1 Function and Localization

• The immunoglobulin lambda constant 1 (IGLC1) gene encodes the constant region of lambda light chains, which pair with heavy chains to form functional antibodies (Immunoglobulin lambda constant 1, Alt Names: Ig lambda chain C region MGC, Ig lambda-1 chain C region).
• Membrane-bound immunoglobulins serve as B-cell receptors; upon antigen recognition, they trigger clonal expansion and differentiation of B lymphocytes into antibody-secreting plasma cells.
• Secreted immunoglobulins mediate the effector phase of humoral immunity, eliminating bound antigens (PubMed:20176268, PubMed:22158414). Related references: PMID:20176268, PMID:22158414
• The antigen-binding site is formed by the variable domains of one heavy and one light chain; V(D)J recombination assembles these domains, and somatic hypermutation after antigen exposure enables affinity maturation (PubMed:17576170, PubMed:20176268). Related references: PMID:17576170, PMID:20176268
• IGLC1 localizes to the extracellular space (secreted) and the cell membrane, as part of B-cell receptors and secreted antibodies.
• Key structural features include an immunoglobulin domain and disulfide bonds; free lambda light chains are associated with Bence-Jones proteins.

Experimental Guidance and Technical Tips

• For flow cytometry applications, titrate the antibody to determine the optimal staining concentration for your cell type and instrument.
• Validate antibody specificity in human B-cell lines or peripheral blood mononuclear cells (PBMCs), using appropriate isotype controls.
• To minimize non-specific binding, consider blocking with normal serum from the host species of the secondary reagent (if used) or with Fc receptor blocking reagents.
• exhibits excitation/emission maxima of 491/516 nm; protect conjugates from prolonged light exposure to preserve fluorescence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, delivering validated monoclonal and polyclonal antibodies, including anti-lambda light chain reagents, to support immunoglobulin research and B-cell studies. Our global supply network provides access to bulk ancillary reagents and can source rare or custom raw materials, helping diagnostic and life science companies accelerate product development. Partner with CamelBio to streamline your procurement from discovery to clinic.