Anti-Human Kappa Light Chain Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P01834

Core Product Specifications and Parameters

Biological Background: Human Kappa Light Chain Function and Localization

• IGKC encodes the constant region of immunoglobulin kappa light chains, a core component of all antibody isotypes that pair with heavy chains to form the antigen-binding fragment (Fab). Related references: PMID:20176268 PMID:22158414
• Membrane-bound antibodies (B cell receptors) trigger B cell activation upon antigen binding, while secreted antibodies neutralize pathogens and mark them for destruction. Related references: PMID:20176268 PMID:22158414
• The antigen-binding site is formed by the variable domains of one heavy and one light chain; diversity is generated by V(D)J recombination and fine-tuned by somatic hypermutation for affinity maturation. Related references: PMID:17576170 PMID:20176268
• IGKC localizes to the cell membrane when part of the B cell receptor complex and is actively secreted into serum and mucosal secretions by plasma cells.
• The protein contains a conserved immunoglobulin domain and a critical disulfide bond that stabilizes the beta-sandwich fold; classified under the immunoglobulin superfamily.
• No tissue-specific expression noted in curated databases; IGKC is widely expressed across B cell lineages and plasma cells.

Experimental Guidance and Technical Tips

• For WB, full-length kappa light chain (~25 kDa) is typically detected under reducing conditions; a ~12 kDa band may appear if only the constant region is present. Include appropriate positive and negative controls.
• For IHC-P, antigen retrieval conditions (e.g., citrate buffer pH 6.0) should be optimized; validate in the relevant tissue.
• The immunogen corresponds to amino acids 1–107 of the constant region, enabling detection of all kappa light chain allotypes regardless of variable domain sequence.
• For ELISA, this antibody can be used as a capture or detection reagent; pairing with an anti-human lambda light chain antibody allows isotype-specific immunoglobulin measurement.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines procurement of this anti-human kappa light chain monoclonal antibody alongside complementary immunoglobulin reagents—validated kappa/lambda antibody pairs, isotype-specific secondary reagents, and bulk ancillary materials—to support your diagnostic assay development, antibody engineering, and B cell research programs from concept to clinic.