Anti-Human Integrin β5/ITGB5 Rabbit Monoclonal Antibody () for Flow Cytometry - P18084

Core Product Specifications and Parameters

Biological Background: Integrin β5/ITGB5 Function and Localization

• Integrin beta-5 (ITGB5) is a transmembrane glycoprotein that heterodimerizes with integrin alpha-V (ITGAV) to form the vitronectin/fibronectin receptor integrin alpha-V/beta-5.
• ITGAV:ITGB5 specifically recognizes the R-G-D tripeptide sequence in fibronectin, mediating cell adhesion, spreading, and migration.
• The receptor plays a role in host-pathogen interactions, serving as a cellular entry receptor for adenovirus type C.
• ITGB5 localizes to the cell membrane via a single-pass transmembrane helix, with a large extracellular domain containing EGF-like repeats.
• Post-translational modifications include phosphorylation and disulfide bond formation, with the protein running at an apparent molecular weight of approximately 88 kDa on SDS-PAGE.
• Keywords associated with ITGB5 include cell adhesion, host-virus interaction, and metal ion binding (calcium and magnesium), which are critical for integrin activation.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular region (aa 24–490), suggesting the antibody (clone ) targets an epitope on the ectodomain; consider using a non-permeabilizing staining protocol for live-cell flow cytometry.
• Include appropriate isotype and fluorescence minus one (FMO) controls to ensure specificity and minimize background.
• Validate expression in your target cell line (e.g., human epithelial or cancer cells) as ITGB5 is widely expressed; coordinate staining with an anti-ITGAV antibody to confirm heterodimer formation if needed.
• Since ITGB5 serves as an adenovirus receptor, this reagent can be used to track viral attachment or entry events when combined with viral particle labeling.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio provides antibodies, antigens, and ancillary reagents to support integrin and cell adhesion research. This anti-ITGB5 conjugate exemplifies our ability to source validated monoclonal antibodies for flow cytometric applications. With a robust supply chain, we offer bulk quantities, customized conjugations, and rare target sourcing to streamline your diagnostic and R&D workflows.