Anti-Human ILT5/CD85a Monoclonal Antibody for Flow Cytometry - O75022

Core Product Specifications and Parameters

Biological Background: ILT5/CD85a Function and Localization

• Protein identity and alternative names: Leukocyte immunoglobulin-like receptor subfamily B member 3 (LILRB3), also known as CD85a, ILT-5, and monocyte inhibitory receptor HL9.
• Immune inhibitory function: Acts as a receptor for class I MHC antigens. Coligation with immune receptors (e.g., FCGR2B and the B-cell receptor) activates LILRB3, leading to down-regulation of antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIMs).
• Expression pattern: Detected in monocytes and B-cells, consistent with its role in innate and adaptive immunity.
• Subcellular localization: Present on the cell membrane, where it functions as a transmembrane inhibitory receptor.
• Structural features: Contains multiple immunoglobulin-like domains, is glycosylated, and undergoes phosphorylation. Alternative splicing generates multiple isoforms.
• Functional keywords: Involved in adaptive immunity, inhibitory receptor activity, and signal transduction. A 3D structure is available for further structural insights.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , a bright fluorophore compatible with flow cytometry (FC). For optimal performance, consider titrating the antibody to determine the best staining concentration for your cell samples.
• Since ILT5/CD85a is expressed on monocytes and B-cells, validate the antibody in relevant human peripheral blood mononuclear cells (PBMCs) or isolated monocyte/B-cell populations to confirm specificity.
• For multiparameter flow cytometry, design appropriate compensation controls when using this antibody with other fluorophores. Gating strategies can be optimized based on the target's membrane expression.
• If using this antibody for functional studies, note that cross-linking may be required to mimic co-ligation-mediated signaling, as LILRB3 functions in conjunction with other immune receptors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to a wide array of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For your immunology and immune-checkpoint research involving ILT5/CD85a, our portfolio supports every stage from concept to clinic, with rapid sourcing of rare-target reagents and expert technical consultation. Partner with CamelBio to accelerate your diagnostic development efficiently.