Anti-Human IL-5 Monoclonal Antibody for Flow Cytometry - P05113

Core Product Specifications and Parameters

Biological Background: Interleukin-5 Function and Localization

• Interleukin-5 (IL-5) is a homodimeric cytokine primarily secreted by T-lymphocytes and NK cells, with a molecular weight of approximately 15.2 kDa.
• It plays a critical role in eosinophil biology, including survival, differentiation, and chemotaxis. Related references: PMID:2653458, PMID:9010276
• IL-5 also acts on activated and resting B-cells, inducing immunoglobulin production, growth, and differentiation.
• Its receptor is composed of the IL5RA subunit and the common beta chain CSF2RB, leading to downstream signaling. Related references: PMID:1495999, PMID:22528658
• Binding triggers activation of kinases including LYN, SYK, and JAK2, propagating signals through the RAS-MAPK and JAK-STAT5 pathways. Related references: PMID:7613138
• IL-5 is a secreted protein and is present in peripheral blood mononuclear cells.
• The protein undergoes alternative splicing and contains disulfide bonds; it is a glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein spanning amino acids 20–134 of human IL-5, which covers the mature protein. This design may enhance specificity for the native cytokine.
• This antibody is designated for intracellular flow cytometry (FC intra). For optimal staining, consider using a fixation/permeabilization protocol that preserves epitope accessibility.
• Since IL-5 is a secreted protein, appropriate stimulation (e.g., PMA/ionomycin) and protein transport inhibitors (e.g., Brefeldin A) may be required to detect intracellular accumulation in T-cells or NK cells.
• Validate the antibody in known positive and negative cell systems, and titrate to determine the optimal concentration for your specific experimental setup.
• The conjugate offers bright fluorescence with a 488 nm excitation, compatible with most flow cytometers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop platform for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research institutions worldwide. For IL-5-related immunoassay development in allergy, asthma, and eosinophilic disorders, CamelBio can supply high-quality reagents and connect you with technical expertise. Whether you need this anti-IL-5 antibody or assistance in sourcing rare targets, CamelBio is your partner from concept to clinic.