Anti-Human IL-4 Monoclonal Antibody () for Intracellular Flow Cytometry - P05112

Core Product Specifications and Parameters

Biological Background: Interleukin-4 (IL-4) Function and Localization

• Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1 (BSF-1), Binetrakin, Lymphocyte stimulatory factor 1, and Pitrakinra, is encoded by the IL4 gene in humans. Alternative splicing generates multiple isoforms.
• IL-4 is a pleiotropic cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils, playing a central role in regulating antibody production, hematopoiesis, and inflammation. Related references: PMID:1993171 PMID:3016727
• IL-4 induces the expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1. Related references: PMID:1993171
• It upregulates the low-affinity IgE receptor (CD23) on lymphocytes and monocytes, contributing to allergic and anti-parasitic responses. Related references: PMID:2521231
• Signaling occurs through two receptor complexes: type 1 (IL4R/IL2RG) on hematopoietic cells and type 2 (IL4R/IL13RA1) on nonhematopoietic cells, leading to JAK3/JAK1 phosphorylation and STAT6 activation. Related references: PMID:10219247 PMID:11526337 PMID:18243101 PMID:7721895
• IL-4 positively regulates IL31RA expression in macrophages and stimulates autophagy in dendritic cells via mTORC1/RUFY4 signaling, influencing T-cell homeostasis.
• In the central nervous system, IL-4 participates in higher cognitive functions such as memory and learning (By similarity).
• The mature secreted glycoprotein has a molecular weight of approximately 17 kDa and contains disulfide bonds essential for its three-dimensional structure.

Experimental Guidance and Technical Tips

• The immunogen covers the mature IL-4 sequence (aa 25-153), targeting the secreted cytokine; ensure proper permeabilization and fixation for intracellular flow cytometry.
• Titrate the conjugate to an optimal concentration for your cell type and stimulation conditions; include isotype controls to assess non-specific binding.
• For rare or weakly expressing cell populations, consider pre-stimulation with PMA/ionomycin or antigen-specific activation and include a viability dye to exclude dead cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic and research workflows. This IL-4 antibody supports intracellular cytokine detection, and our sourcing platform can also fulfill your needs for rare target reagents, recombinant proteins, and custom conjugation services—bridging every stage from concept to clinic.