Anti-Human IL-1β Monoclonal Antibody () for Intracellular Flow Cytometry - P01584

Core Product Specifications and Parameters

Biological Background: IL-1β Function and Localization

• IL-1β is a potent pro-inflammatory cytokine that induces multiple effects including prostaglandin synthesis, neutrophil influx, T- and B-cell activation, and fibroblast proliferation; it was initially identified as the major endogenous pyrogen (PubMed:3920526).
  Related references: PMID:3920526
• It promotes Th17 differentiation and synergizes with IL-12 to stimulate IFN-γ production from Th1 cells (PubMed:10653850).
  Related references: PMID:10653850
• IL-1β contributes to angiogenesis by upregulating VEGF synergistically with TNF and IL-6 (PubMed:12794819).
  Related references: PMID:12794819
• During pyroptosis, mature IL-1β is released extracellularly through gasdermin-D (GSDMD) pores, linking inflammasome activation to inflammation (PubMed:33377178, PubMed:33883744).
  Related references: PMID:33377178, PMID:33883744
• It serves as a sensor for S. pyogenes infection; bacterial SpeB protease cleaves and activates IL-1β, triggering a protective inflammatory response (PubMed:28331908).
  Related references: PMID:28331908
• Subcellularly, IL-1β localizes to the cytoplasm, lysosomes, and is secreted via exosomes; it is expressed primarily in activated monocytes/macrophages.
• Keywords: Cytokine, inflammatory response, mitogen, pyrogen, secreted.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is designed for intracellular flow cytometry (FC intra). For detection of intracellular IL-1β, cells should be fixed, permeabilized, and may require pretreatment with a protein transport inhibitor (e.g., brefeldin A) to block secretion and retain the cytokine in the Golgi/cytoplasm.
• The immunogen corresponds to the mature form of human IL-1β (amino acids 117-269), suggesting that the antibody recognizes the processed active cytokine. Validate staining in activated human monocytes/macrophages or IL-1β-expressing cell lines.
• As a directly conjugated primary antibody, no secondary reagent is needed; follow standard intracellular staining protocols with appropriate isotype controls.
• Cross-reactivity is reported for human; if using in other species, perform sequence homology analysis and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing for diagnostic manufacturers and research laboratories. This anti-IL-1β monoclonal antibody supports inflammation, immunology, and infectious disease research, and CamelBio can assist with customized reagent supply and technical consultation to accelerate assay development.