Anti-Human IL-17A Monoclonal Antibody for Intracellular Flow Cytometry - Q16552

Core Product Specifications and Parameters

Biological Background: IL-17A Function and Localization

• IL-17A (interleukin-17A), also known as CTLA-8, is a founding member of the IL-17 cytokine family, encoded by the IL17A gene (GeneID: 3605; SWISS: Q16552). It is primarily expressed in memory Th17 cells.
• Core effector function: IL-17A signals through the IL17RA-IL17RC heterodimeric receptor, activating NF-kB and MAPK pathways to induce expression of pro-inflammatory cytokines, chemokines, and matrix metalloproteinases (PubMed:17911633, PubMed:18684971, PubMed:19825828). Related references: PMID:17911633 PMID:18684971 PMID:19825828
• Host defense: Critical for neutrophil recruitment and antimicrobial peptide production (e.g., beta-defensins) in response to extracellular bacteria and fungi (PubMed:24120361, PubMed:21350122). Related references: PMID:24120361 PMID:21350122
• Epithelial integrity: Maintains mucosal barrier function by regulating tight junction proteins such as OCLN, and promotes production of antimicrobial beta-defensins (By similarity).
• Germinal center regulation: Enhances B cell retention, somatic hypermutation, and plasma cell differentiation in secondary lymphoid organs (By similarity).
• Antiviral roles: Enhances T cell cytotoxicity against West Nile virus and supports B cell-mediated antibody responses during influenza infection (By similarity).
• Subcellular localization: Secreted; contains a signal peptide and a disulfide-bonded glycoprotein structure.
• Additional features: Undergoes homodimerization, and its activity is synergistically enhanced by IL-17F.

Experimental Guidance and Technical Tips

• The immunogen (aa 24-155) corresponds to the mature secreted form of human IL-17A after signal peptide cleavage, ensuring recognition of the biologically active cytokine.
• Intracellular flow cytometry application requires cell fixation and permeabilization; optimize fixation/permeabilization conditions for your specific cell type and stimulation protocol.
• Use appropriate positive and negative controls (e.g., stimulated vs. unstimulated Th17 cells) to confirm staining specificity.
• Store upon arrival at 2-8°C and protect from light; avoid repeated freeze-thaw cycles to preserve fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, offering validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This IL-17A antibody supports immunology and inflammation research, reflecting our commitment to providing high-quality tools for diagnostic development from concept to clinic.