Anti-Human IgM Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01871

Core Product Specifications and Parameters

Biological Background: Human IgM Function and Localization

• Encodes the immunoglobulin heavy chain constant mu (IgM) region, which exists as both a membrane-bound B cell receptor (BCR) complex and a secreted antibody essential for humoral immunity. Related references: PMID:20176268, PMID:22158414
• The variable domain is assembled through V(D)J recombination and somatic hypermutations, enabling affinity maturation for specific antigens. Related references: PMID:17576170, PMID:20176268
• Secreted IgM (sIgM) multimerizes into high-order polymers (mainly pentamers, occasionally hexamers), providing multivalent, high-avidity antigen recognition. Related references: PMID:32029689, PMID:37095205
• Natural sIgM is polyreactive and recognizes conserved self- and pathogen-derived structures, while immune sIgM is antigen-specific and induced upon pathogen exposure.
• Mediates effector functions by binding the high-affinity Fc receptor FCMR on lymphoid cells to promote neutralizing IgG responses, and by recruiting C1q to activate the classical complement pathway. Related references: PMID:12847249, PMID:19006321, PMID:28230186
• Involved in mucosal immunity: transported across mucosal epithelium by PIGR, and IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across M cells toward antigen-presenting cells. Related references: PMID:32029689
• Membrane-bound IgM provides constitutive tonic signaling for B cell survival and mediates pre-BCR signaling that regulates B cell selection and allelic exclusion.
• Subcellular localization includes secreted forms and cell membrane; the protein is a glycoprotein with intrachain disulfide bonds and alternative splicing isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 218–453 within the constant region of human IgM, which may allow detection of both secreted and membrane-bound forms.
• For Western blot (WB), the predicted molecular weight is 49 kDa under reducing conditions; however, confirm the actual band size using appropriate sample preparation and controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate antibody specificity using human lymphoid tissue samples (e.g., tonsil or spleen) as positive controls.
• For ELISA, consider pairing with a suitable capture antibody or direct detection based on the assay format; assess linearity and sensitivity with known IgM standards.
• Since cross-reactivity is reported only for human, verify reactivity with other species if required before use in multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with streamlined access to high-quality IVD raw materials, from validated antibody pairs and bulk ancillary reagents to rare target sourcing. This anti-human IgM monoclonal antibody supports humoral immunity research, including B cell development, complement activation, and mucosal defense studies. Contact our team for custom bulk orders, technical consultation, and end-to-end reagent supply solutions tailored to your assay development needs.