Anti-Human IgM (FC) Rabbit Monoclonal Antibody for WB, IF/ICC, FC, ELISA - P01871

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Mu (Human IgM Heavy Chain) Function and Localization

• Protein Identity and Gene: Immunoglobulin heavy constant mu (IgM heavy chain) is encoded by the IGHM gene. Alternative names include Ig mu chain C region BOT, GAL, and OU.
• Immunoglobulin Heavy Chain Function: As a constant region of immunoglobulins, it plays a central role in humoral immunity, forming membrane-bound receptors that trigger B cell activation and secreted antibodies that eliminate antigens. Affinity maturation is achieved via somatic hypermutation. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Multimeric Assembly of Secreted IgM: The constant region of secreted IgM (sIgM) multimerizes into pentamers and occasionally hexamers, conferring high avidity for polyvalent antigen binding. Related references: PMID:32029689 PMID:37095205
• Effector Functions of sIgM: sIgM activates the classical complement pathway by recruiting C1q and promotes phagocytosis of apoptotic cells and pathogens through interactions with Fc receptors and mannose-binding lectin. Related references: PMID:12847249 PMID:19006321 PMID:28230186
• Membrane-Bound IgM in BCR Complex: The membrane-bound form constitutes the B cell receptor (BCR), delivering tonic signals for B cell survival and mediating pre-BCR selection and allelic exclusion.
• Subcellular Localization: IgM is found both secreted into extracellular fluids and tethered to the cell membrane, consistent with its dual role as a humoral effector and surface receptor.
• Structural and Post‑Translational Features: The protein contains immunoglobulin domains, undergoes glycosylation and disulfide bond formation, and is a target of alternative splicing. Direct protein sequencing and three‑dimensional structures are available.

Experimental Guidance and Technical Tips

• The immunogen spans the Fc region (aa 218–453) common to all human IgM isoforms; the antibody is therefore expected to react with both secreted and membrane‑bound IgM.
• For Western blotting under reducing conditions, the IgM heavy chain migrates at approximately 75 kDa due to glycosylation, although the predicted unmodified weight is 49 kDa. Validate the band shift in your lysate.
• In immunofluorescence/ICC, surface IgM can be detected on live B cells; permeabilization may reveal intracellular pools. Optimize fixation and permeabilization for your cell type.
• Flow cytometry: this antibody is suitable for staining B cell surface IgM. Titrate the antibody and include appropriate isotype controls.
• ELISA: use this antibody as a detection reagent for human IgM in sandwich immunoassays. Cross‑reactivity with other species should be checked if extending to non‑human samples.
• Always validate the antibody in the relevant sample system under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw material partner, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for diagnostic development. Our anti‑human IgM (FC) monoclonal antibody supports research into humoral immunity, autoimmune disorders, and infectious disease serology. With our one‑stop sourcing service, we help diagnostic manufacturers bridge from concept to clinic, ensuring reliable supply and technical consultation.