Anti-Human IgG4/Immunoglobulin heavy constant gamma 4 Rabbit Polyclonal Antibody for WB and ELISA - P01861

Core Product Specifications and Parameters

Biological Background: Immunoglobulin heavy constant gamma 4 Function and Localization

• Immunoglobulin heavy constant gamma 4 (IGHG4) is the constant region of the gamma-4 heavy chain, defining the IgG4 isotype.
• Igs, including IgG4, serve dual roles as membrane-bound B-cell receptors and secreted antibodies, driving clonal expansion, affinity maturation, and effector functions (PubMed:17576170, PubMed:20176268, PubMed:22158414). Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Isotype switching to IgG4 occurs via class-switch recombination in activated B cells (PubMed:17576170, PubMed:20176268, PubMed:22158414). Related references: PMID:17576170, PMID:20176268, PMID:22158414
• The IgG4 constant region lacks the ability to trigger antibody-dependent cellular cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC).
• IgG4 plays a key role in immune tolerance by blocking IgE-mediated allergic responses and regulating mast cell degranulation.
• In tumor microenvironments, IgG4 may contribute to immunosuppressive mechanisms.
• The protein is secreted and also expressed on the B-cell membrane.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation.
• UniProt annotates the molecular weight at 43832 Da (~44 kDa).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50-150 of human IGHG4, targeting a sequence within the constant region.
• This antibody is established for Western blotting (WB) and ELISA; optimization of dilutions and conditions is recommended for each assay format.
• For WB, consider using the predicted molecular weight of ~44 kDa as a reference for band identification.
• Cross-reactivity with homologous IgG4 from other species may occur due to sequence conservation, but validation has been limited to human.
• The antibody can be employed as a detection or capture reagent in ELISA-based assays for IgG4 quantification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD and research raw materials, supporting your workflow from concept to clinic. This anti-human IgG4 polyclonal antibody is part of a broader portfolio that includes validated antibody pairs, monoclonal/polyclonal tools, and bulk ancillary reagents for humoral immunity and immunoglobulin studies. For customized or hard-to-find targets, our team facilitates efficient procurement and technical consultation to accelerate your diagnostic development.