Anti-Human IgG3 Rabbit Monoclonal Antibody for WB, ELISA - P01860

Core Product Specifications and Parameters

Biological Background: Human IgG3 Function and Localization

• Human IgG3 is the constant region of the immunoglobulin gamma-3 (IgG3) heavy chain, belonging to the immunoglobulin family.
• Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes that act as receptors in humoral immunity.
• Secreted immunoglobulins, known as antibodies, block pathogen interactions and elicit effector functions via their constant region. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The antigen-binding region’s affinity is generated through V-(D)-J rearrangement, somatic hypermutation, and affinity maturation. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• B cells undergo class-switch recombination to change constant regions, switching from IgM/IgD to IgG, IgA, or IgE. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• IgG3 constant region interacts with Fc receptors and the complement system to mediate antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, complement-dependent cytotoxicity (CDC), and inflammatory responses.
• The protein localizes to the cell membrane and can be secreted; alternative splicing produces multiple isoforms.
• Key post-translational modifications include glycosylation and disulfide bond formation; the protein contains immunoglobulin-like domains, a transmembrane helix (in membrane-bound form), and repeat regions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 99–167 of the human IgG3 constant region, targeting a sequence that is common to both membrane-bound and secreted forms of the protein.
• For Western blotting, test under both reducing and non-reducing conditions. The observed protein weight may vary (41–49 kDa) due to glycosylation; the unmodified predicted molecular weight is approximately 49 kDa.
• Because of high sequence homology among IgG subclasses, the antibody may cross-react with other human IgG subtypes. Validate specificity using appropriate isotype controls and purified proteins.
• For ELISA applications, the antibody can be used for detection of human IgG3 in biological samples. Consider pairing with a capture antibody against a non-overlapping epitope for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials—from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents and rare target raw-material supply. For research on human IgG3 and humoral immunity, we support your assay development and scale-up needs with expert technical consultation tailored to the IVD industry.