Anti-Human IgG3 Polyclonal Antibody for WB and ELISA - P01860

Core Product Specifications and Parameters

Biological Background: Human IgG3 Function and Localization

• IGHG3 encodes the immunoglobulin heavy constant gamma 3 (IgG3) chain, also known as HDC or Ig gamma-3 chain C region, a member of the immunoglobulin superfamily responsible for humoral adaptive immunity.
• IgG3 serves dual roles as a membrane-bound B-cell receptor and as a secreted antibody. Upon antigen binding, it mediates clonal B-cell expansion and differentiation into antibody-secreting plasma cells. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The constant region of IgG3 (Fc) engages innate immune effector cells and the complement system, driving antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, complement-dependent cytotoxicity (CDC), and pro-inflammatory responses. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Through class-switch recombination, B cells can replace the default IgM/IgD constant region with IgG3, tailoring the antibody isotype to specific effector functions during an immune response. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• IgG3 is localized both as a secreted protein in extracellular fluids and as a transmembrane receptor on the surface of B lymphocytes.
• Key structural features include immunoglobulin domains, a transmembrane helix, multiple disulfide bonds, and N-linked glycosylation, which are essential for protein folding, stability, and effector interactions.
• The IgG3 heavy chain contains repeat regions in the constant domain, distinguishing it from other IgG subclasses and contributing to its extended hinge and increased flexibility.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 80–180 of the human IgG3 constant region, a segment conserved among reported IgG3 alleles. Verify sequence alignment when using non-human samples or engineered constructs.
• For Western blot, separation under reducing conditions is recommended. A band near 41 kDa is expected for the target constant region fragment; validate with appropriate molecular weight markers and a positive control (e.g., human serum or purified IgG3).
• In ELISA, use direct or indirect coating with the recombinant immunogen or native human IgG3 to capture the antibody. Optimize coating concentrations and blocking conditions for your plate format and detection system.
• This antibody is reported reactive with human IgG3. Cross-reactivity with other IgG subclasses or immunoglobulins from other species has not been tested; confirm specificity in your assay matrix using isotype‑matched controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality immunological reagents. This anti‑human IgG3 polyclonal antibody supports the development of assays for humoral immunity profiling, isotype quantitation, and B‑cell biology studies. Beyond single antibodies, CamelBio offers bulk ancillary reagents, validated antibody pairs for sandwich immunoassays, and customized sourcing of rare‑target antibodies, helping you accelerate from concept to clinic with a single, reliable partner.