Anti-Human IgG1 (Fc) Monoclonal Antibody for WB, IF-P, ELISA - P01857 / P01859 / P01860 / P01861

Core Product Specifications and Parameters

Biological Background: Human IgG1 Heavy Chain Constant Region Function and Localization

• Immunoglobulin heavy constant gamma 1 (IGHG1) encodes the constant region of the IgG1 heavy chain, a major component of human antibodies. It pairs with light chains to form the antigen-binding structure of immunoglobulin molecules.
• The constant region defines the isotype and mediates effector functions. As a membrane-bound receptor on B lymphocytes, it triggers clonal expansion and differentiation into plasma cells upon antigen binding. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Secreted IgG1 antibodies neutralize pathogens and engage Fc receptors on innate immune cells, leading to antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and complement-dependent cytotoxicity (CDC). Related references: PMID:17576170 PMID:20176268 PMID:22158414
• Class-switch recombination in B cells replaces the IgM constant region with IgG1, diversifying effector functions while preserving antigen specificity established by V(D)J recombination and affinity maturation. Related references: PMID:17576170 PMID:20176268 PMID:22158414
• The protein is found on the cell membrane as part of the B cell receptor complex and is secreted into the extracellular space by plasma cells.
• Post-translational modifications include conserved disulfide bonds linking heavy and light chains, and glycosylation at specific residues critical for effector function and structural stability.
• Additional keywords highlight involvement in adaptive immunity, chromosomal rearrangement, alternative splicing, and a transmembrane helix in the membrane-bound form.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the Fc region (amino acids 100–330) of human IgG1 (P01857). This domain is highly conserved among IgG subclasses, so the antibody may also recognize IGHG2 (P01859), IGHG3 (P01860), and IGHG4 (P01861). Validate specificity in your experimental system if subclass discrimination is required.
• For Western blotting, the expected band is around 44 kDa under reducing conditions. Optimize loading controls and blocking agents to minimize background from abundant serum immunoglobulins in complex samples.
• In IF-P, signal may localize to B lymphocytes or plasma cells. Include appropriate negative controls and consider antigen retrieval if staining fixed tissue.
• For ELISA, this antibody can be used as a detection or capture reagent for human IgG1 Fc. Pair with appropriate standards and confirm linearity in your assay matrix.
• Since the antibody is raised in rabbit, choose anti-rabbit secondary reagents carefully to avoid cross-reactivity with endogenous human IgG in direct detection formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a reliable one-stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for immunoglobulin studies. Our global sourcing network ensures consistent availability of this anti-human IgG1 Fc antibody and related molecules, accelerating your development from concept to clinic. Whether you need bulk raw materials or technical consulting for rare target applications like human IgG subclass profiling, CamelBio is your trusted partner.