Anti-human IgG (Fc) Monoclonal Antibody for IHC-P, ELISA - P01857 / P01859 / P01860 / P01861

Core Product Specifications and Parameters

Biological Background: Immunoglobulin Heavy Constant Gamma 1 (IgG1) Function and Localization

• Immunoglobulin heavy constant gamma 1 (IgG1) constitutes the constant region of immunoglobulin gamma heavy chains, forming the backbone of IgG antibodies.
• Membrane-bound IgG serves as the B cell receptor; antigen binding triggers clonal expansion and differentiation into antibody-secreting plasma cells. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Secreted IgG antibodies mediate humoral effector functions: neutralization of pathogens, opsonization, and activation of the complement cascade via the Fc region. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Class-switch recombination diversifies Ig constant regions, enabling B cells to switch from IgM/IgD to IgG, IgA, or IgE, thereby tailoring effector responses. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• The Fc region of IgG1 engages Fcγ receptors on innate immune cells to elicit antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis.
• IgG1 is both secreted into bodily fluids and displayed on the B cell membrane, consistent with its dual role in humoral immunity.
• Structurally, IgG1 features immunoglobulin domains stabilized by disulfide bonds and contains glycosylation sites critical for stability and effector function.
• UniProt keywords: Adaptive immunity, cell membrane, disulfide bond, glycoprotein, secreted, transmembrane, alternative splicing, chromosomal rearrangement.

Experimental Guidance and Technical Tips

• For IHC-P, consider antigen retrieval optimization and test dilution series on known human IgG-positive tissues (e.g., tonsil, spleen) to determine optimal working concentration.
• In ELISA, the antibody can be used to detect or capture human IgG; validate in appropriate buffer systems and confirm specificity against purified IgG subclasses.
• The immunogen is a synthetic peptide from human IgG Fc region; be aware that multiple UniProt accessions (P01857, P01859, P01860, P01861) suggest cross-reactivity with IgG1, IgG2, IgG3, and IgG4 subclasses.
• Perform proper controls, including isotype-matched negative controls and known positive and negative samples, to confirm labeling specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For human IgG-related research and immunoassay development, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets. Contact us to streamline your immunoglobulin detection workflows with reliable starting materials.