Anti-Human IgA1 Monoclonal Antibody () for Flow Cytometry - P01876

Core Product Specifications and Parameters

Biological Background: IgA1 Function and Localization

• IgA1 is the major immunoglobulin class in mucosal secretions, providing the first line of antigen-specific defense at mucosal surfaces. Related references: PMID:2241915
• As part of the humoral immune response, secreted IgA1 mediates effector functions that eliminate bound antigens and pathogens. Related references: PMID:20176268, PMID:22158414
• The antibody's antigen-binding specificity arises from variable domains assembled via V(D)J recombination, which can undergo somatic hypermutation to achieve high-affinity binding. Related references: PMID:17576170, PMID:20176268
• IgA1 antibodies are expressed both as membrane-bound B cell receptors and as secreted soluble proteins, localizing to the cell surface and extracellular spaces.
• Post-translational modifications such as glycosylation and disulfide bond formation are essential for IgA1 stability and function.
• Alternative splicing of the IGHA1 gene generates different isoforms, and chromosomal rearrangements have been associated with certain hematologic malignancies.

Experimental Guidance and Technical Tips

• The immunogen (aa 99-353) targets the constant region of IgA1 heavy chain, likely minimizing cross-reactivity with other immunoglobulin isotypes; validate specificity in your assay system.
• For flow cytometry, determine the optimal antibody titer by titration and include appropriate isotype controls and Fc receptor blocking to reduce non-specific binding.
• Ensure single-cell suspensions and viability gates are used for accurate IgA1 surface staining on B lymphocytes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw material sourcing, specializing in immunoglobulin research tools including this anti-IgA1 conjugate. We support diagnostic manufacturers with validated monoclonal antibodies, custom conjugations, and bulk supply, ensuring seamless integration from concept to clinical application. Count on CamelBio to accelerate your mucosal immunity assay development.