Anti-Human IgA Rabbit Monoclonal Antibody for WB and ELISA - P01876 / P01877

Core Product Specifications and Parameters

Biological Background: IgA Function and Localization

• Immunoglobulin heavy constant alpha 1 (IGHA1) is the constant region of the alpha heavy chain of IgA1 antibodies, encoded by the IGHA1 gene on chromosome 14.
• IgA is the major immunoglobulin class in mucosal secretions, providing primary defense against pathogens at mucosal surfaces. Related references: PMID:2241915
• Membrane-bound IgA serves as B-cell receptor, triggering B-cell activation, clonal expansion, and differentiation into plasma cells upon antigen binding.
• Secreted IgA mediates effector functions of humoral immunity, including neutralization, agglutination, and opsonization to eliminate antigens. Related references: PMID:20176268, PMID:22158414
• The variable domain of the heavy chain combines with the variable domain of the light chain to form the antigen-binding site, with affinity maturation achieved through V(D)J recombination and somatic hypermutation. Related references: PMID:17576170, PMID:20176268
• IGHA1 undergoes alternative splicing to produce membrane-bound and secreted isoforms; the secreted form lacks the transmembrane domain.
• Localizes to the cell membrane as part of the B-cell receptor and is secreted into extracellular spaces and body fluids.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, essential for stability and effector function.

Experimental Guidance and Technical Tips

• For Western blot, consider running samples under reducing conditions to detect the ~60 kDa heavy chain; non-reducing conditions may reveal higher molecular weight complexes.
• In ELISA, the antibody may be used as capture or detection reagent; validate pairing with appropriate secondary antibodies and confirm specificity using purified human IgA.
• The recombinant immunogen spans the full constant region, suggesting the antibody may recognize both IgA1 and IgA2; cross-reactivity with other immunoglobulin classes should be experimentally excluded.
• Antibody performance may vary across sample types; optimize incubation conditions and use appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to supply a comprehensive range of IVD raw materials. For immunoglobulin research and IgA-based assay development, we offer validated monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents. Our sourcing expertise also extends to rare targets, ensuring uninterrupted supply from concept to clinic.