Anti-Human Ig Kappa Chain Monoclonal Antibody for WB, ELISA - P01834

Core Product Specifications and Parameters

Biological Background: Immunoglobulin kappa constant Function and Localization

• Immunoglobulin kappa constant (IGKC) is the constant region of the immunoglobulin kappa light chain, essential for antibody structure and function.
• Antibodies are membrane-bound receptors on B cells or secreted glycoproteins; binding to antigen triggers B cell clonal expansion and differentiation into antibody-secreting plasma cells, mediating humoral immunity (PubMed:20176268, PubMed:22158414). Related references: PMID:20176268 PMID:22158414
• The antigen-binding site is formed by one heavy and one light chain variable domain; these are generated by V-(D)-J recombination and can undergo somatic hypermutation for affinity maturation upon antigen exposure (PubMed:17576170, PubMed:20176268). Related references: PMID:17576170 PMID:20176268
• IGKC is present at the cell membrane as part of the B cell receptor and is also secreted into the extracellular space.
• Post-translational modifications include disulfide bond formation, critical for maintaining the immunoglobulin domain structure.
• UniProt classifies IGKC with keywords: 3D-structure, adaptive immunity, direct protein sequencing, disease variant, proteomics identification, and reference proteome.
• Multiple allotypic variants of the kappa constant region exist (e.g., Km(1), Km(2), Km(3)), encoded by the IGKC gene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length constant region (amino acids 1-107), ensuring recognition of all IGKC allotypes and both membrane-bound and secreted forms.
• For Western blotting, the expected band size is approximately 12 kDa; validate in human serum or B cell lysates to confirm specificity.
• For ELISA, pairing with a heavy chain-specific antibody may enable detection of intact antibodies or free light chains; test across relevant human biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD antibodies and raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for immunodiagnostic development. For humoral immunity research or clinical assay development targeting human immunoglobulin kappa chain, CamelBio simplifies procurement with a one-stop sourcing solution.