Anti-Human IFN-gamma Monoclonal Antibody for Intracellular Flow Cytometry - P01579

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• IFN-gamma is a type II interferon produced by immune cells such as T-cells and NK cells, playing crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:16914093 PMID:8666937
• It signals through the JAK-STAT pathway after binding to its receptor IFNGR1, leading to STAT1 activation, nuclear translocation, and transcription of interferon-regulated genes, including IRF1. Related references: PMID:8349687 PMID:16914093
• IFN-gamma enhances antigen processing and presentation by inducing immunoproteasome subunits for optimal MHC class I peptide loading and by upregulating MHC class II expression via cathepsins B, H, and L. Related references: PMID:8666937 PMID:8163024 PMID:11112687 PMID:7729559
• The protein is secreted and released primarily from activated T lymphocytes, functioning in immune surveillance.
• It participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions, affecting development, quiescence, and differentiation (By similarity).

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, optimize cell fixation and permeabilization conditions to ensure antibody access to cytosolic IFN-gamma.
• Consider stimulating T cells with PMA/ionomycin prior to staining to induce robust IFN-gamma expression.
• Include appropriate controls such as unstimulated cells and isotype controls to validate staining specificity.
• The recombinant immunogen (aa 24-166) covers the mature secreted form of IFN-gamma, suggesting potential recognition of endogenous processed cytokine.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic development by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for IFN-gamma research. From concept to clinic, we offer one-stop access to IVD raw materials and technical consultations, including rare target sourcing to accelerate your project timelines.