Anti-Human IFN-gamma Monoclonal Antibody for Flow Cytometry (Intracellular) - P01579

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• Interferon gamma (IFN-gamma), also known as immune interferon, is a type II interferon cytokine that plays central roles in antimicrobial, antiviral, and antitumor immune responses.
• It is produced primarily by activated T lymphocytes and NK cells upon immune stimulation. Related references: PMID:16914093 PMID:8666937
• IFN-gamma signals through the JAK-STAT pathway after binding to its receptor IFNGR1, leading to STAT1 activation, nuclear translocation, and transcription of downstream target genes. Related references: PMID:8349687 PMID:16914093
• It enhances antigen presentation by inducing immunoproteasome subunits and the activator PA28, thereby increasing the quantity, quality, and repertoire of peptides for MHC class I loading. Related references: PMID:8666937 PMID:8163024 PMID:11112687
• IFN-gamma up-regulates MHC class II complexes by promoting the expression of cathepsins B, H, and L, key molecules for antigen processing. Related references: PMID:7729559
• It participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions, affecting their quiescence and differentiation.
• Subcellular location: Secreted.
• Keywords: Antiviral defense, Cytokine, Glycoprotein, Pharmaceutical, 3D-structure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature IFN-gamma protein sequence (aa 24-166), suggesting the antibody may recognize both endogenous and recombinant human IFN-gamma. Consider validating performance in your specific sample type and assay conditions.
• For intracellular flow cytometry (FC intra), typical protocols involve cell stimulation with PMA/ionomycin in the presence of a protein transport inhibitor (e.g., Brefeldin A), followed by fixation and permeabilization. Optimize stimulation time and antibody concentration for optimal signal-to-noise.
• Titrate the antibody from 0.1 to 1 µg per test to determine the optimal staining index. Include unstimulated and isotype controls to assess background and specificity.
• This product is supplied in liquid format with ice bag shipping; upon receipt, aliquot and store as recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, bulk ancillary reagents, and tailored sourcing solutions from concept to clinic. This anti-human IFN-gamma mAb targets a key cytokine in immune activation and Th1 response, making it highly relevant for diagnostic developers working on immune monitoring, infectious disease, or cancer immunotherapy assays. Whether you need matched detection antibodies or custom bulk conjugates, we help streamline your supply chain with consistent, high-quality materials.