Anti-Human IDO1 Monoclonal Antibody () for Flow Cytometry (Intracellular) - P14902

Core Product Specifications and Parameters

Biological Background: IDO1 Function and Localization

• IDO1 (indoleamine 2,3-dioxygenase 1) is a heme-containing oxidoreductase that catalyzes the initial and rate-limiting step of tryptophan catabolism via the kynurenine pathway. Related references: PMID:17671174 PMID:18026683
• It plays a central role in peripheral immune tolerance by depleting tryptophan and producing catabolites that inhibit T-cell proliferation and promote regulatory T-cell differentiation, thereby preventing autoimmunity and immunopathology. Related references: PMID:25691885
• IDO1 suppresses anti-tumor immunity, facilitating immune evasion in the tumor microenvironment; its overexpression in tumors is associated with poor clinical outcomes. Related references: PMID:14502282 PMID:23103127 PMID:25157255 PMID:18418598
• By limiting tryptophan availability, IDO1 restricts the growth of intracellular pathogens, acting as a defense mechanism against certain infections. Related references: PMID:25691885
• The enzyme protects the fetus from maternal immune rejection at the maternal-fetal interface, contributing to pregnancy maintenance. Related references: PMID:25691885
• IDO1 is predominantly localized to the cytosol and is inducible in most tissues, with high expression in mature dendritic cells of lymphoid organs, placental endothelial cells, and certain epithelial cells. Related references: PMID:25691885 PMID:18418598

Experimental Guidance and Technical Tips

• This -conjugated rabbit monoclonal antibody is designed for intracellular flow cytometry on human samples. Fix and permeabilize cells using standard protocols prior to staining.
• IDO1 expression is inducible by stimuli such as IFN-γ; consider stimulating target cells to upregulate IDO1 for improved detection sensitivity.
• Titrate the antibody to determine the optimal concentration for your specific experimental system, balancing signal intensity and background.
• Use appropriate isotype controls and compensation controls when incorporating into multi-color flow cytometry panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Beyond this anti-IDO1 antibody, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support immunology and oncology workflows. From concept to clinic, we streamline your procurement with reliable, B2B-focused supply solutions.