Anti-Human ICAM-1/CD54 Monoclonal Antibody for Flow Cytometry - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• Functions as a receptor ligand for ITGAL:ITGB2/LFA-1, enabling leukocyte cell-cell adhesion and providing a calibrated system to adjust T-cell killing strength based on antigen stimulation. Related references: PMID:3086451, PMID:3340213, PMID:38195629
• Also acts as a ligand for ITGAM:ITGB2/MAC-1, mediating adhesion between stimulated neutrophils and stimulated endothelial cells. Related references: PMID:1980124
• During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Related references: PMID:17875742
• Promotes cell aggregation in epithelial cells via interaction with MUC1. Related references: PMID:11173916
• Acts as a receptor for major group rhinovirus A and B capsid proteins, as well as Coxsackievirus A21 capsid proteins, facilitating viral entry.
• Targeted for degradation by the Kaposi's sarcoma-associated herpesvirus/HHV-8 E3 ubiquitin ligase MIR2, presumably to evade immune cell lysis.
• Localizes to the cell membrane and is expressed on non-hematopoietic cells (vascular endothelium, thymic epithelium, fibroblasts) and hematopoietic cells (tissue macrophages, stimulated T lymphoblasts, dendritic cells). Expressed at low levels on peripheral blood leukocytes. Related references: PMID:3086451
• Undergoes post-translational modifications including disulfide bond formation, N-linked glycosylation, and phosphorylation; also subject to ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 28–480 of the extracellular domain, ensuring recognition of native surface ICAM-1.
• This conjugate is optimized for flow cytometry; titrate the antibody in your specific assay system to determine the optimal working concentration.
• For best results, use human cell lines or primary cells known to express ICAM-1 (e.g., HUVECs, Jurkat T cells stimulated with mitogen).
• Avoid repeated freeze-thaw cycles; store according to shipping conditions (ice bag) upon receipt.

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CamelBio provides diagnostic manufacturers and research labs with integrated access to IVD raw materials, technical services, and consulting. Our portfolio includes validated monoclonal/polyclonal antibodies, antibody pairs, and ancillary reagents for targets such as ICAM-1/CD54 in immune monitoring applications. Contact us to streamline your procurement from discovery to commercialization.