Anti-Human IARS (Isoleucyl-tRNA Synthetase) Polyclonal Antibody for WB, ELISA - P41252

Core Product Specifications and Parameters

Biological Background: Isoleucine--tRNA ligase, cytoplasmic Function and Localization

• Isoleucine--tRNA ligase, cytoplasmic (IARS) is a member of the class I aminoacyl-tRNA synthetase family, also known as isoleucyl-tRNA synthetase (IRS) or IleRS.
• Catalyzes the specific attachment of isoleucine to its cognate tRNA(Ile) in a two-step reaction: activation of isoleucine by ATP to form Ile-AMP, followed by transfer to the tRNA acceptor end, a critical step in protein biosynthesis.
• Encoded by the IARS1 gene (GeneID: 3376) in humans.
• Primarily localized in the cytoplasm and cytosol.
• Subject to post-translational modifications including acetylation and phosphorylation (as identified by proteomics).
• Clinically, mutations in IARS1 have been linked to intellectual disability (disease variant).
• Expressed at the protein level in liver and muscle tissues.
• Predicted molecular weight is ~144.5 kDa (UniProt P41252), consistent with the observed band at 144 kDa.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (aa 1158–1262) of human IARS; verify that the antibody recognizes the expected epitope in your assay format.
• For Western blotting, expect a band near 144 kDa; use high-molecular-weight markers and optimize transfer conditions for large proteins.
• As a polyclonal antibody, lot-to-lot consistency should be validated, and species cross-reactivity limited to human; test in other species if required.
• For ELISA applications, titrate the antibody against the recombinant protein or cell lysate to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized polyclonal antibodies (such as this IARS pAb), bulk ancillary reagents, and rare target raw-material supply. For protein biosynthesis and translation research, our portfolio includes critical tools for detecting aminoacyl-tRNA synthetases and related machinery. Let CamelBio bridge your procurement needs from concept to clinic with reliable, scalable solutions.