Anti-Human HBG1/2 Rabbit Monoclonal Antibody for WB, IF-P, and ELISA - P69891 / P69892

Core Product Specifications and Parameters

Biological Background: HBG1 Function and Localization

• Hemoglobin subunit gamma-1 (HBG1), also designated gamma-1-globin or Hb F Agamma, is one of the two gamma-globin chains that, together with alpha-globin, form fetal hemoglobin F (HbF).
• According to UniProt, the primary function of HBG1 is to provide the gamma chain component of fetal hemoglobin, which is crucial for oxygen transport during fetal development.
• HBG1 is specifically expressed in red blood cells, consistent with its role in oxygen delivery.
• The protein features several notable characteristics: it binds a heme prosthetic group with an iron ion, enabling reversible oxygen binding; and it undergoes post-translational modifications including acetylation, phosphorylation, and S-nitrosylation (UniProt keywords).
• The molecular weight of the mature HBG1 chain is 16,128 Daltons (UniProt), matching the observed 16 kDa band in SDS-PAGE.
• Alternative names for this protein include Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, and Hemoglobin gamma-A chain.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 68–147 of human HBG1 (P69891). This region is highly identical between HBG1 and HBG2, supporting dual recognition.
• For Western blot, use reducing conditions; a band at approximately 16 kDa is expected. Positive control: fetal or cord blood lysates.
• In immunofluorescence on paraffin sections, optimize antigen retrieval and validate on HbF-positive tissues (e.g., fetal liver).
• For ELISA, test both direct and sandwich formats; pairing with an anti-HBG capture antibody may enhance sensitivity.
• Cross-reactivity is reported as human; if other species are needed, perform an in-house validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to high-quality IVD raw materials, including this anti-HBG1/2 monoclonal antibody, to accelerate your fetal hemoglobin research and diagnostic kit development. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your project from concept to clinical application with reliable supply and technical consultation.